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Órgano oficial de la Facultad de Odontología, UNAM

2018, Number 1

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Rev Odont Mex 2018; 22 (1)

Assessment of cytology techniques in oral mucosa of Sjögren's syndrome patients

Busamia B, Gobbi C, Albiero E, Yorio M

Language: Spanish
References: 16
Page: 30-34
PDF size: 219.22 Kb.


ABSTRACT

The aim of the present research project was to compare two techniques for oral cytology study (exfoliative cytology and impression cytology) in order to assess changes in oral mucosa which might allow non -invasive diagnosis of Sjögren’s syndrome cases (SS). Patients: 50 patients were selected, patients were paired by age and gender, and had been diagnosed with Sjögren’s syndrome according to criteria of the American-European Consensus. Patients were distributed into the following three experimental groups: Healthy control group (C), n = 14, Dry mouth and eyes group without SS, (ME) n = 13, and SS group n = 23. Material and methods: A cell harvesting brush (Cytobrush) was used for the exfoliative cytology procedure, sliding it along a glass plate and later fixating harvested cells in 95% ethanol. Cellulose acetate paper (Millipore Hawp 304®) was used for the impression cytology procedure. The paper was in 1 cm long stripes which were placed on the oral mucosa surface above upper vestibular groove; stripes were immobilized and pressure was applied for three seconds. Papanicolau (PAP) technique was used for dyeing. Morphology and histomorphology were assessed studying the following: cytoplasmic area (CA), nuclear areas (NA) nucleus-cytoplasm relationship (N:C) and amount of cells per square millimeter (mm2). Results: Both techniques revealed the following in C: isolated single-layered epithelial cells, basophils, normal central nuclei, 20 to 30 per mm2, N:C ratio 1:8. In the eye and mouth group (EM): grouped and folded isolated cells, cytoplasm with eosinophilic predominance increase of cell amount to 40 per mm2, nucleus-cytoplasm relationship N/C 1:4. SS patients showed the following: nuclear area with denser chromatin, 400 cells per mm2, and 1:2 N/C relationship with respect to mouth and ears and control. Statistically significant differences wereobserved among groups in all studied characteristics. Conclusion: We can infer that impression cytology can be used in systemic and oral lesion’s diagnosis in patients afflicted with hyposalivation.


REFERENCES

  1. Garcia-Carrasco M, Fuentes-Alexandro S, Escarcega RO, Salgado G, Riebeling C, Cervera R. Pathophysiology of Sjögren’s syndrome. Arch Med Res. 2006; 37 (8): 921-932.

  2. Mavragani CP, Moutsopoulos NM, Moutsopoulos HM. The management of Sjögren’s syndrome. Nat Clin Pract Rheumatol. 2006; 2 (5): 252-261.

  3. Vitali C, Bombardieri S, Jonsson R, Moutsopoulos HM, Alexander EL, Carsons SE et al. Classification criteria for Sjögren’s syndrome: a revise version of the European criteria proposed by the American-Europea Consensus Group. Ann Rheum Dis. 2002; 61 (6): 554-558.

  4. Per SR, Scully C, Hegartty AM. An update of the etiology and management of xerostomia. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97 (1): 28-46.

  5. Sule Y, Ebru T, Muge B, Ronca M, Sezen F. Comparative analysis of autoantibodies against alfa-fodrin in serum, tear fluid, and saliva from patients with Sjögren’s syndrome. J Rheumatol. 2006; 33 (7): 1289-1292.

  6. Busamia B, Gonzalez-Moles MA, Mazzeo M, Linares J, Demarchi M, Gobbi C et al. Assessing the determination of salivary electrolytes and anti-Ro and anti-La antibodies for the diagnosis of Sjögren’s syndrome (SS). Med Oral Patol Oral Cir Bucal. 2010; 15 (3): e437-440.

  7. Egbert PR, Lauber S, Maurice DM. A simple conjunctival biopsy. Am J Ophthalmol. 1977; 84 (6): 798-801.

  8. Sawada Y, Yuan C, Huang AJ. Impression cytology in the diagnosis of acanthamoeba keratitis with surface involvement. Am J Ophthalmol. 2004; 137 (2): 323-328.

  9. Verges RC, Pita SD. Estudio de la población de células caliciformes en los síndromes de ojo seco mediante un método citológico atraumático. Arch Soc Esp Oftalmol. 1988; 55 (5): 525-530.

  10. Mehrotra R, Gupta A, Singh M, Ibrahim R. Application of cytology and molecular biology in diagnosing premalignant or malignant oral lesions molecular. Mol Cancer. 2006; 5: 11.

  11. Maksem JA, Weldmann J. Specialized Preparative devices are not needed for liquid-based thin-layer cytology: an alternate manual method using a metastable alcoholic gel. Diagn Cytopathol. 2001; 25 (4): 262-264.

  12. Kavatkar AN, Nagwanshi CA, Dabak SM. Study of a manual method of liquid-based cervical cytology. Indian J Pathol Microbiol. 2008; 51 (2): 190-194.

  13. Singh R, Joseph A, Umapathy T, Tint NL, Dua HS. Impression cytology of the ocular surface. Br J Ophthalmol. 2005; 89 (12): 1655-1659.

  14. Rodríguez MF, Juyo A, Rojas A. Descripción de la metaplasia escamosa por citología de impresión en pacientes con ojo seco. Ciencia y Tecnología para la Salud Visual y Ocular No 8: 51-62/ Enero-Junio de 2007.

  15. Tseng SC. Staging of conjunctival squamous metaplasia by impression cytology. Ophthalmology. 1985; 92 (6): 728-733.

  16. Nelson JD, Wright JC. Conjunctival goblet cell densities in ocular surface disease. Arch Ophthalmol. 1984; 102 (7): 1049-1051.




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