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Órgano oficial de la Facultad de Odontología, UNAM

2017, Number 4

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Rev Odont Mex 2017; 21 (4)

Evaluation of two RNA extraction methods in children’s saliva

Vidal MAM, Suárez CA

Language: Spanish
References: 31
Page: 245-252
PDF size: 232.87 Kb.


ABSTRACT

Objective: To standardize an RNA (ribonucleic acid) extraction protocol in children’s saliva specimens. Methods: The study was conducted on saliva specimens from 60 children who participated in the study with their parents’ authorization. Comparison of two RNA extraction methods was established; methods assessed concentration, quality and yield. Moreover, genic expression of GUSB gene was achieved with RT-PCR (reverse transcriptase-polymerase chain reaction). Data were analyzed through measurements of central tendency and dispersion, frequencies and percentages. T-Student and χ2 tests were used in order to differentiate between both methods (p ‹ 0.05). Results: Analysis of cell amounts per saliva ml revealed a mean of 564,977.8 (SD = 246,678.6); a RNA in children’s saliva extraction method was standardized, specifically the method using RNeasy® Protect Saliva Mini Kit Qiagen exhibited better characteristics of RNA concentration (p = 0.0000) and yield (p = 0.0000) when compared to the method using QIAzol®; no RNA (p = 0.146) quality differences were found. Conclusions: RNA could be extracted from children’s saliva specimens, therefore, it is suggested to use saliva for the molecular analysis of different oral and systemic diseases.


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