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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2005, Number 2

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Rev Biomed 2005; 16 (2)

Biology and diagnostic methods for dengue.

Acosta-Bas C, Gómez-Cordero I

Language: Spanish
References: 226
Page: 113-138
PDF size: 180.24 Kb.


ABSTRACT

At present the diagnosis of dengue is one of the priorities in the sphere of diagnosis, due to the high incidence of this re-emerging disease. In this work a review is done on the current state of knowledge about the general characteristics of the virus dengue (its structures, composition and viral expression, viral genome and antigenic determinants). The structural and not structural proteins are detailed with a special emphasis, as well as their role in the immune response, the identification of the immunogenic elements and the definition of the epitopes of proteins involved in the immunologic response against dengue. The general characteristics of the assays for the detection of viral antigens are presented and the development of isolation techniques and viral typification are detailed, as well as polymerase chain reaction techniques used for the detection of viral RNA and antigens, by means of ELISA techniques, using novel amplification methods. This paper discusses the antecedents of the serologic studies and the general characteristics of them. In the same way this work deals with the advantages and disadvantages of the serologic diagnosis techniques available in their different formats. Tendencies in the development of new diagnostic methods, based on the recombinant technology and chemical synthesis, are analyzed with the objective of increasing the sensitivity and specificity of the assays used for the detection of specific antibodies.


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