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ISSN 1405-1710 (Print)

2017, Number 2

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Rev Cent Dermatol Pascua 2017; 26 (2)

Platelet-rich plasma: comparative study of four protocols for its production

Pérez-Montesinos G, Medina-Bojórquez A, Hernández-Ramírez H, Morales-Sánchez MA, Peralta-Pedrero ML, Jurado-Santa CF

Language: Spanish
References: 15
Page: 41-44
PDF size: 189.67 Kb.


ABSTRACT

In the search for tissue regeneration, tissue engineering has introduced the use of growth factors. In recent times the use of platelet rich plasma (PRP) has started to take advantage of the factors contained in their granules. However, there are various systems to obtain them non-standardized. The aim of the present study was to compare the efficacy of 4 centrifugation protocols to obtain PRP. In a first phase 8 protocols described previously in the literature were tested in a standard laboratory centrifuge system. The 4 protocols with the highest platelet count were selected, being tested again in 30 patients with a baseline blood count. The highest number of platelets were obtained with the protocol of 1 single centrifugation at 100 g for 8 minutes (p ‹ 0.001, 95% CI), the lowest leucocyte count corresponded to the protocol of 2 centrifugations at 400 g for 10 minutes each. The purity decreased by lowering the value of the relative centrifugal force. The lowest cell count was obtained with the protocol with shorter centrifugation. The results obtained differ significantly with the figures reported with similar protocols and commercial procurement systems. Studies with a higher level of evidence are needed to standardize this technique.


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