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ISSN 1027-2852 (Electronic)
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2016, Number 4

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Biotecnol Apl 2016; 33 (4)

Micropropagation of garlic (Allium sativum L.) and determination of the genetic stability of the plantlets obtained by AFLP markers

Izquierdo-Oviedo H, Disotuar R, González MC, González SJ

Language: English
References: 40
Page: 4211-4218
PDF size: 364.73 Kb.


ABSTRACT

This work was aimed to establish a methodology for the micropropagation of garlic (Allium sativum L.) ‘Criollo-9’ clone, to assess different combinations of AFLP primers to determine the genetic stability of vitroplants and to assess morphoagronomically the regenerants that were obtained in vitro. Cloves showing no signs of disease and under asepsis conditions were selected; the caudal apices were extracted between 5-10 mm and growth and development were assessed, both in vitro and ex vitro. As results, with the MS medium with NAA (0.1 mg/L) and Kinetin (0.1 mg/L) the cauline apices reached a higher percentage of establishment, in a shorter time; the highest number of shoots (20) was emitted in a medium enriched with NAA (0.1 mg/L) and 2-iP (4 mg/L) and for the first time in Cuba, AFLP markers were used in garlic and with the different combinations of primers that were used variability was not detected in the regenerants obtained. The percentage of in vitro bulbification was higher and was achieved in a shorter time in MS medium without growth regulators. The acclimatization of the microbulbils was higher than 99 % in a substrate containing zeolite (25 %) and organic matter (75 %). Under field conditions, yields higher than 12 t/ha were obtained and the results with molecular markers were corroborated, so the micropropagation technique is feasible to apply to this garlic genotype.


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