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ISSN 1561-3046 (Electronic)

2016, Number 2

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Rev Cub Med Mil 2016; 45 (2)

Diagnosis of american trypanosomiasis in students of the Latin American School of Medicine

Delgado OJP, Montoto CD, Dean ÁV, Núñez FFÁ, Mora GSR, Fraga NJ

Language: Spanish
References: 40
Page: 119-130
PDF size: 144.77 Kb.


ABSTRACT

Introduction: countries where Chagas disease is not endemic (CD) confront, among their challenges, the determination of its prevalence in endemic area people.
Objectives: determine the presence of anti-Trypanosoma cruzi antibodies by different serological methods in serum samples of students from the Latin American School of Medicine (ELAM), detect the presence of the parasite genome by polymerase chain reaction (PCR-kDNA) in the studied group and evaluate, preliminarily, the methods used in the working algorithm for the diagnosis of Chagas disease in Cuba.
Methods: a cross-sectional descriptive study of ELAM students was conducted from September to November 2012. These students underwent serological and molecular studies for the diagnosis of CD, due to the research carried out by the Ministry of Public Health (MINSAP) To Cuba, using four commercial methods of serological diagnosis, with parallel use of the PCR-kDNA technique.
Results: seropositivity, using serological methods, was 6.25%, and this parasite was detected in five seropositive students. The presence of the parasite genome (diagnostic sensitivity and specificity: 100%) was shown for the PCR-kDNA technique. The diagnostic sensitivity and specificity, found in this preliminary study, allow it to be considered a useful tool to diagnose CD in patients with chronic indeterminate phase. The serological and molecular methods of the algorithm to corroborate CD used in this research showed sufficient validity, diagnostic reliability and concordance to justify its use at each of the confirmation levels.


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