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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2017, Number 1

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Rev Mex Patol Clin Med Lab 2017; 64 (1)

Bacteriological study of the pediatric patient with probable diagnosis of tuberculosis

García-González R, Nájera-Garduño MC, Arzate-Barbosa P, Reyes TA

Language: Spanish
References: 57
Page: 36-42
PDF size: 382.97 Kb.


ABSTRACT

Tuberculosis is an infectious disease transmitted mainly through airways and considered a public health problem. The spread of Mycobacterium tuberculosis from highly smear-positive patients is decisive in children infected considered the reservoir of future cases of tuberculosis, so his diagnosis in these patients, it is essential for detection in order to provide the correct treatment and timely. Although there are simple techniques, accurate diagnosis and effective treatment with the diagnosis of tuberculosis in pediatric patients it is difficult. The symptoms, the data found during physical examination are nonspecific and insignificant in the mild and moderate forms of the disease. In this situation, the account information that pediatric patient is limited which makes this be forgotten, for its non-infectious nature, not having been considered or no risk to public health programs. The aim of this work was to determine the presence of BAAR in pediatric patients in orden to confirm the clinical diagnosis. The methodology used was to obtain samples from respiratory, phenotypic determination of M. tuberculosis (Ziehl-Neelsen, culture in liquid and solid media and biochemical identification) and genotypic determination with the use of chain reaction (PCR). The results obtained were 13.4% for smear positivity, 43% for cultivation and 57% for PCR. Conclusions: Of the three tests applied smear was the least sensitive and PCR was the highest, but there is little difference from the crop.


REFERENCES

  1. The Global Plan to Stop TB 2011-2015. Transforming the fight towards elimination to tuberculosis. World Health Organization. 2010. ISBN 978 924 1500340.

  2. Global tuberculosis control 2010. Geneve 2010 (WHO/HTM/TB/2010.7)

  3. Starke JR. Transmission de Mycobacterium tuberculosis to and from children and adolescens. Semin Pediatr Infect Dis. 2001, 12: 115-123.

  4. Global tuberculosis report 2012. World Health Organization 2012.

  5. Kochi A. The global tuberculosis situation and the new control strategy of the World Health Organization. Tubercle. 1991; 72: 1-6.

  6. Manual para el diagnóstico bacteriológico de la tuberculosis. Normas y Guía técnica. Organización Mundial de la Salud. Primera parte, 2008.

  7. Farga V. Tuberculosis. Segunda edición. Publicaciones técnicas Mediterráneo. Santiago de Chile. 2012.

  8. Mukherjee A, Lodha R, Kabra SK. Changing trends in childhood tuberculosis. Indian J Pediatr. 2011; 78 (3): 328-333. doi: 10.1007/s12098-010-0298-4

  9. Zumla A. Algunas acciones factibles contra la tuberculosis. BMJ. Edición Latinoamericana. 1999; 7: 151-152.

  10. Cernoch PL, Enns PK, Saubolle MA, Wallace Jr RL. Laboratory diagnosis of the Mycobacterioses. CUMITECH 16. ASM Press. 1994.

  11. Global Tuberculosis Report 2014. World Health Organization 2014.

  12. Moreno PD, Martín AA, Gómez NA, Baquero-Artigao F, Escribano Montaner KF, Gómez-Pastrana DI et al. Diagnóstico de la tuberculosis en la edad pediátrica. Documento de consenso de la Sociedad Española de Infectología Pediátrica (SEIP) Y la Sociedad Española de Infectología Pediátrica (SENP) An Pediatr (Barc). 2010; 73: 143e1-143e14.

  13. Guiadance for national TB programmes of tuberculosis in childrens. WHO ATM/TB/2006.71

  14. Singh V. Diagnosis of childhood tuberculosis-the road so far and the way ahead. Pediatric Infectious Disease. 2010; 2 (3): 119-122.

  15. Oberhelman R, Soto-Castellares G, Gilman RH, Caviedes L, Castillo ME, Kolevic E et al. Diagnostic approaches for paediatric tuberculosis by use de different specimen types, culture methods, and PCR a prospective case-control study. Lancet Infect Dis. 2010; 10: 612-620.

  16. Lighter J, Rigaud M. Diagnosing childhood tuberculosis. Curr Peobl Pediatr Adolesc Health Care. 2009; 39: 61-88.

  17. Chin PD, Crane CM, Diul MY, Sun SJ, Agraz R, Taylor S et al. Spread of Mycobacterium tuberculosis in a community implementing recommended elements of tuberculosis control. JAMA. 2000; 283: 2968-2974.

  18. Sang-Nae Cho. Tuberculosis: diagnostics. Tuberculosis. 2007; 87: S14-S17.

  19. García de Lomas J, Navarro D. New directions in diagnostics. Pediatric Infect Dis J. 1997; 16 (3 Suppl): S43-48.

  20. Borroughs M, Beitela A, Kawamura A, Revai K, Ricafort R, Chiu K et al. Clinical presentation of tuberculosis in culture-positive children. Pediatric Infect Dis. 1999; 18 (5): 440-446.

  21. Iselman LS. Tuberculosis in children: un update. Pediatric pulmonary. 1996; 21: 201-220.

  22. Abadco DL, Steiner P. Gastric lavage is better than bronchoalveolar lavege for isolation of Mycobacterium tuberculosis in childhood pulmonary tuberculosis. Pediatr Infect Dis. 1992. 11: 735-738.

  23. Pai M, Minion J, Sohn H, Zwerling A, Perkins MD. Novel and improved technologies for tuberculosis diagnosis: progress and challenges. Clin Chest Med. 2009; 30:701-716.

  24. Blancarte ML, Anzaldo de JG y Balandrano de ES. Manual de técnicas y procedimientos de laboratorio en tuberculosis. Publicación técnica del INDRE. 1992. No. 20, México, D.F.

  25. Castillo VA. Valor diagnóstico de los criterios de Stegen modificado por Toledo en la tuberculosis infantil en el Hospital Nacional Guillermo Almera Irigoyen. Revista Peruana de Pediatría. 2005; 58: 30-39.

  26. Manual para el diagnóstico bacteriológico de la tuberculosis. Normas y Guía Técnica II Parte. Organización Panamericana de la Salud. 2008.

  27. Palacios JJ, Ferro J, Telentin M, Roces SG, Prendes P, García JM et al. Comparison of solid and liquid cultura media with the polimerasa chain reaction for detection of Mycobacterium tuberculosis in clinical samples. Eur J Clin Microbiol Dis. 1996; 15: 478-483.

  28. Wilson MD. Rapid Diagnosis of Mycobacterium tuberculosis Infection and drug susceptibility testing. Arch Pathol Lab Med. 2013; 137: 812-819.

  29. Pai M, Kalanteri S, Dheda K. New tools and emerging technologies for the diagnosis of tuberculosis: Part II. Active tuberculosis and drug resistance. Expert Rev Mol Diagn. 2006; 6: 423-432.

  30. Soini H, Musser JM. Molecular diagnosis of Mycobacteria. Clin Chem. 2001; 45: 809-814.

  31. García GR, Lara HA, Nájera GMC, Arzate BP. Detección de Mycobacterium tuberculosis en niños, con empleo de dos sistemas de cultivo y su confirmación con la reacción en cadena de la polimerasa. Rev Enf Infec en Pediatía. 1999; XII: 323-328.

  32. Díaz ML, Herrera T, López VY, Calva JJ, Hernández R, Ruíz PG et al. Polimerase chain reaction for detection of Mycobacterium tuberculosis DNA in tissue and assessement of its utility in the diagnosis of hepatic granulomas. J Lab Clin Med. 1996; 127: 359-363.

  33. Thierry D, Cave MD, Eisenach K, Crawford JT, Bates JH, Gicquel B et al. IS6110, as IS-like element of Mycobacterium tuberculosis complex. Nucleic Acids Res. 1990; 18: 188.

  34. Sambrook J, Fritsc EF and Maniatis T. Molecular Cloning. A laboratory Manual Vol. 3, Second Edition. Cold Spring Harbor laboratory. Pres. New York, 1989.

  35. Smith KC, Starke JR, Eisenach K, Org LT and Denby M. Detection of Mycobacterium tuberculosis in clinical specimens from children using Polimerase chain reaction. Pediatric. 1996; 97: 155-160.

  36. Eisenach KD, Sifford MD, Cave MD, Bates JH, Crawford JT. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am Rev Respir Dis. 1999; 144: 1160-1163

  37. Fisker C y Hass D. Micobacteria: Tuberculosis y lepra. En 5ª edición Mecanismos de las enfermedades infecciosas. Engleberg NC, DiRita V, Dermody T (ed). Editorial Wolter Kluwer/Lipeincot Wilkins. Baltimore. 2013. pp. 257-268.

  38. Yates TY, Khan PY, Knight GM, Taylor JG, McHugh TD, Lipman M, Moore DA. The transmission of Mycobacterium tuberculosis inhigh burden setting. Lancet Infect Dis. 2016; 16: 227-238. doi: 10.1016/S1473-3099(15)00499-5.

  39. Tarago A, Jiménez S, Sánchez A, Arroyo A, Aracil J y cols. Brote de tuberculosis en un colegio de primaria: descripción y consideraciones sobre el valor del jugo gástrico en el manejo de microepidemias. Enferm Infecc Clin. 2011; 29: 90-95.

  40. Cruz Anleu ID, Velásquez Serrato JR. Tuberculosis infantil. ¿Cómo diagnosticarla? Arch Argent Pediatr. 2012; 110: 144-151.

  41. Murthy S, Keystone J, Kissoon N. Infections of the developing world. Crit Care Lin. 2013; 29: 485-507.

  42. Sudre P, Ten Dam G, Kochi A. Tuberculosis: a global overview of the situation today. Bull World Health Organ. 1992; 70: 149-159.

  43. Corbett EL, Watt CJ, Walker N, Maher D, Williams BG. The growing burden of tuberculosis. Arch Intern Med. 2003; 163: 1009-1021.

  44. Ghanashyam B. Childhood tuberculosis-the time for action is NOW. Pediatric Infectious Disease. 2012; 4: 94-95.

  45. Gómez PD, Torrenteras R, Caro P, Anguita ML, Barrio AML, Andrés A et al. Diagnosis of tuberculosis in children using a polymerase chain reaction. Pediatric Pulmonalogy. 1999; 28: 344-351.

  46. Montenegro SH, Gilman RH, Sheen P, Cama R, Caviedes L, Hopper T et al. Improved Detection of Mycobacterium tuberculosis in Peruvian Children by Use of a Heminested IS6110 Polymerase Chain Reaction Assay. Clin Infect Dis. 2003; 36: 16-23.

  47. Merino JM, Álvarez T, Marrero M, Ansó S, Elvira A, Iglesias G et al. Microbiology pediatric primary pulmonary tuberculosis. CHEST Journal. 2001; 119: 1434-1438.

  48. Baghaei P, Tabarsi P, Farnia P, Radaei AH, Kazempour M, Faghani YA et al. Utility of gastric lavage for diagnosis of tuberculosis in patients who are unable to expectorate sputum. Glob Infect Dis. 2011; 3:339-343. doi:10.4103/0974-777X.91054

  49. Karam BJ. Diagnóstico y tratamiento de tuberculosis durante la infancia. Revista Mexicana de Puericultura y Pediatría. 1994; 1: 117-124.

  50. Chakravorty S, Dudeja M, Hanif M, Tyagi JS. Utility of universal samples processing methodology, combining smear microscopy, culture, and PCR for diagnosis of tuberculosis. J Clin Microbiol. 2005; 43: 2703-2708.

  51. Jacob JT, Mehta AK, Leonard MK. Acute forms of tuberculosis in adults. Am J Med. 2009; 122: 12-17.

  52. Lincon EM, Harris LC. Endobronchial tuberculosis in children, a study of 156 patients. Am Rev Tuber. 1958; 77: 39-61.

  53. Berean K, Roberts FJ. The reliability of acid fast stained smears of gastric aspirate specimens. Tubercle. 1988; 69: 205-208.

  54. Magee J, Freeman R, Barrett A, Attrill H, Lightfoot NF. Testing for tuberculosis. The Lancet. 1996; 347: 476.

  55. Venkataraman B, Vasudevan M, Grupta A. A new microarray platform for whole-genome expression profiling of Mycobacterium tuberculosis. J Microbiological Methods. 2014; 97: 34-43.

  56. Benjamin WH, White KB, Moser SA. The MB/BacT is a sensitive method of gastric aspirate specimens in laboratory with a low rate of isolation. J Clin Microbiol. 2000; 38: 3133-3134.

  57. Benson CA. Mycobacterium avium-intracellulare Comples Disease. In: Schlossberg D (Ed). Tuberculosis and nontuberculosis mycobacterial infectious. WB. Saunders Company. 4th Edition. Philadelphia Pennsylvania. 1999. 351-371.




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