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2016, Number 1

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Rev Cubana Farm 2016; 50 (1)

Evaluation of cytotoxicity of different marine extracts in a cell line panel

Rodríguez-Sánchez H, Díaz-García A, Frión-Herrera Y, Monzote FL

Language: Spanish
References: 21
Page: 127-138
PDF size: 287.26 Kb.


ABSTRACT

Introduction: natural extracts from marine sources represent an important source for the discovery of new compounds with anti-carcinogenic potentialities.
Objectives: To determine the effect of five extracts from several marine organisms pm the viability of a panel of five cell lines (A549, HEp-2, MDA-MB-231, SiHa y MRC- 5).
Methods: the effects of the extracts (Physalia physali, Cassiopea xamachana, Tripneustes ventricosus, Echinometra lucunter) were then determined by using the colorimetric assay with 3 (4,5 dimethyl-2-tiazoil)/2,5-difeniltetrazolium bromide. Additionally, the effect of extract of Echinometra lucunter was determined on the expression of apoptotic genes p53, survivin, bcl-xL and noxa in SiHa.
Results: all the extracts affected the cell viability of the normal cell line MRC-5 of the human lung. However, viability of tumoral cell lines did not decrease except for the extract from Echinometra lucunter. This extract just affected the viability of tumor cell line SiHa. The mean inhibitory concentrations (IC50) showed that only for Echinometra lucunter extract, the tumor cell line SiHa revealed a IC50 of 52.07 ± 11 µg/mL that is significantly lower than that of MRC-5 with IC50 of 98.6 ± 14 µg/mL, therefore the selectivity against the tumor cells was shown. Moreover, the extract markedly decreased the expression of propapoptosis genes, thus indicating the cell death from necrosis in SIHa cells.
Conclusions: extract from Echinometra lucunter was selective against tumor cells SiHa. Other experiments that will include other cervix uterine cancer cell lines can confirm the potential of this extract to have an effect on this histological cancer type.


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