Diaz-Uribe CE, Oliveros G, Muñoz-Acevedo A, Vallejo LWA

Language: English
References: 24
Page: 359-368
PDF size: 200.30 Kb.

ABSTRACT

Introduction: antioxidant activity is the capacity of a substance to inhibit oxidative degradation, mainly through its ability to react with both radical and non-radical species (e.g. singlet oxygen). Interest by scientific communities in the study of the antioxidant capacity of natural compounds has increased in recent years, due to their possible applications in the pharmaceutical, cosmetic and food industries.
Objective: estimate the antioxidant capacity of naringin against singlet oxygen using the rubrene method.
Methods: naringin was isolated from peels of the fruit of bitter orange (Citrus aurantium) and characterized using several spectroscopic techniques (UV-Vis and FTIR). The global rate constant for the reaction of ¹O₂ with naringin was determined with the Stern-Volmer plot derived from a stationary kinetic state based on the competition reaction with rubrene.
Results: results showed that naringin acted as singlet oxygen quenching agent with a global rate constant of 2.1 x 10⁷ M^-1s^-1 (derived from the linear relationship of Stern-Volmer).
Conclusion: the kinetic study conducted suggests that naringin could be used as a singlet oxygen quenching agent in biological systems to protect them from oxidative damage.

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