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2016, Number S2

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Gac Med Mex 2016; 152 (S2)

Level of Expression of Gene CTSL and its Correlation with Natural Killer T-Cells in Mexican Pediatric Patients with Recent-Onset Type 1 Diabetes

Gómez-Díaz RA, Medina-Santillán R, Castro-Magdonel BE, Bekker-Méndez C, Gómez-Zamudio J, Nishimura-Meguro E, Garrido-Magaña E, Lizárraga-Paulin L, Aguilar-Herrera BE, Valladares-Salgado A, Cruz M, Mondragón-González R, Ortiz-Navarrete V, Wacher NH

Language: Spanish
References: 33
Page: 14-21
PDF size: 123.12 Kb.


ABSTRACT

Objective: To compare the level of expression of the gene CTSL and its correlation with NKT cells in patients with recentonset type 1 diabetes (T1D), their siblings, and healthy controls. Methods: Analytical cross-sectional design. Patients with T1D ‹ 3 months evolution, their siblings, and healthy controls were included. Percentages and absolute numbers of NKT cells were measured with expression of the CTSL gene. Results: 124 subjects: with T1D (n = 48), siblings (n = 44) and controls (n = 32) were included. HbA1c was greater and C-peptide lower in T1D than the other groups and sibling age was higher (p ‹ 0.001). There were no differences in NKT cells between T1D (0.176 ± 0.202) and controls (0.118 ± 0.133), but the percentage was higher in siblings (0.246 ± 0.188; p = 0.002). Lower level of expression of the CTSL gene associated with both absolute number (r: 0.4607; 95% CI: –0.08425 to –0.7935; p = 0.043) and percentage of NKT cells (r: 0.4540; 95% CI: –0.0927 to –0.7903; p = 0.045) in the T1D group. Conclusions: Patients with T1D have lower percentage and absolute number of NKT cells compared to their siblings. NKT cells absolute numbers are correlated with the expression of CTSL in T1D patients.


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