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ISSN 0864-4551 (Print)

2016, Number 2

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Biotecnol Apl 2016; 33 (2)

P3, a monoclonal antibody capable to activate B-1a cells

Martínez D, Cabrera L, Hernández AM

Language: English
References: 45
Page: 2211-2216
PDF size: 927.37 Kb.


ABSTRACT

P3 is a murine IgM mAb that recognizes N-glycolylated gangliosides, glycolipids expressed on the cell membranes of most vertebrates. It also binds other self-antigens like sulfatides and has high homology with an antibody that recognizes myelin oligodendrocytes. Previously it was demonstrated that P3 mAb is able to activate an idiotypic cascade involving autologous anti-idiotypic B and T cells, in the absence of adjuvant or carrier protein, despite being a self-protein. The anti-P3 idiotype response is mediated not only by CD4+, but also by CD8+ T cells. B-1a cells are the dominant B cell population during the early phases of development, when the idiotypic connectivity is established. Germline natural antibodies secreted by these cells play fundamental roles fighting infections and keeping the homeostasis. In this work, we show that the P3 mAb was able to recognize B-1a cells from naïve BALB/c mice. This antibody induced the overexpression of the activation markers CD25, CD69 and CD86, and increased the secretion of IgM and a mixed pattern of IFN-γ, IL-4 and IL-10 cytokines by B-1a cells. The P3 mAb ability of activating the B-1a cells could contribute to its immunogenicity in the syngeneic model, further supporting its use as a tool to specifically target and stimulate B-1a cells.


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