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2016, Number 1

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Biotecnol Apl 2016; 33 (1)

A homologous DNA recombination pathway alternative to RecFOR and RecBCD induced by gamma irradiation in Salmonella typhimurium

Cuétara E, Sánchez-Lamar A, Montero-Montoya J, Espinosa-Aguirre J, Camacho-Carranza R

Language: English
References: 61
Page: 1201-1207
PDF size: 367.61 Kb.


ABSTRACT

Understanding how cells repair DNA damage is a key issue in molecular and cell biology. Ionizing radiation induces DNA damage mainly repaired by a universal mechanism, homologous recombination (HR). Since DNA is chemically the same in all organisms and repair machinery is highly conserved, bacterial models are suitable for assaying genotoxicity, due to their plasticity and low cost. Traditionally, the assays that measure HR use double-strand ends to initiate the recombination events, favoring the RecBCD pathway. The present work was aimed to evaluate gamma rays-induced HR in Salmonella typhimurium, in terms of segregation rates by means of the duplication-segregation assay (seg-dup), which does not favor any particular DNA repair pathway. RecA-independent recombination events were detected at high doses of gamma radiation (150 Gy), which were partially dependent on RecB, SbcCD and RecQ. The seg-dup assay was efficient to elucidate the proteins involved in repairing radiation induced injury. Moreover, due to its simplicity, low costs and the versatility of S. typhimurium strains available, it could be useful to assess the mechanism of action of novel drugs that exert their action by interacting with cell HR machinery.


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