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2016, Número 1

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Biotecnol Apl 2016; 33 (1)


Ruta de recombinación homóloga del ADN alternativa a RecFOR y RecBCD inducida por radiación gamma en Salmonella typhimurium

Cuétara E, Sánchez-Lamar A, Montero-Montoya J, Espinosa-Aguirre J, Camacho-Carranza R

Idioma: Ingles.
Referencias bibliográficas: 61
Paginas: 1201-1207
Archivo PDF: 367.61 Kb.


RESUMEN

La comprensión sobre cómo las células reparan los daños en el ADN es un aspecto esencial para la biología molecular y celular. Los daños inducidos por la radiación ionizante se reparan a través de un mecanismo universal, conocido como recombinación homóloga (RH). El ADN es químicamente homogéneo entre los organismos y su maquinaria de reparación es altamente conservada, por l que los modelos bacterianos son apropiados para estudiar la genotoxicidad, dada su mayor plasticidad y bajo costo. Tradicionalmente, los ensayos que miden la RH emplean segmentos terminales de doble cadena para iniciar los eventos recombinatorios, y favorecen la ruta de RecBCD. En este trabajo se estudió RH inducida por radiación gamma en Salmonella typhimurium, según su tasa de segregación evaluada mediante el ensayo de segregación-duplicación (seg-dup). Este ensayo no favorece a ninguna ruta de recombinación. Se detectaron eventos de recombinación independientes de RecA, inducidos por altas dosis de radiación gamma (150 Gy), y parcialmente dependientes de recB SbcCD y RecQ. El ensayo seg-dup dilucidó de forma eficiente cuáles fueron las proteínas involucradas en la reparación del daño inducido por la radiación. Dada la simplicidad y los bajos costos del ensayo, y la versatilidad de las cepas de S. typhimurium generadas, estas pudieran permitir la evaluación del mecanismo de acción de fármacos cuya acción esté mediada por la interacción con la maquinaria celular de RH.


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