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2004, Number 2

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Vet Mex 2004; 35 (2)

Repellent effect of Melinis minutiflora extract on Boophilus microplus tick larvae

Muro CFJ, Cruz-Vázquez C, Fernández-Ruvalcaba M, Molina TJ

Language: English/Spanish
References: 26
Page: 153-159
PDF size: 157.40 Kb.


ABSTRACT

The objective of the present study was to investigate the repellence effect of M. minutiflora plant extracts on B. microplus larvae, and to identify the chemical compounds present in the extracts. The effect was evaluated by a repellence bioassay in an olfactometer using extracts from stems, leaves and whole plants treated with different organic solvents such as methanol, chloroform, hexane and acetone. The identification of the chemical compounds was carried out by a gas chromatography-mass spectrometry procedure using whole plant extract in acetone. The study demonstrated that all extracts evaluated showed a repellence effect that ranged from 43 to 90%. The best repellence percentages were observed from extracts obtained using acetone as the extraction solvent. Twelve chemical compounds were identified, the highest relative abundance corresponded to eicosane (18.53%), followed by methyl ester linolenic acid (16.08%) and hexadecanoic acid (14.20%). However, it is possible that the repellence effect was influenced by the additive action of all the compounds identified.


REFERENCES

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  2. Kunz SE, Kemp DH. Insecticides and acaricides: Resistance and environmental impact. Rev Sci Tech 1994;13:1249-1286.

  3. Kaaya GP. Non chemical agents and factors capable of regulating tick populations in nature: a mini review. Insect Sci Appl 1992;13:587-594.

  4. Kaaya GP. The potential for anti-tick plants as component of an integrated tick control strategy. Ann NY Acad Sci 2000;916:576-582.

  5. Thompson KC, Roa E, Romero N. Anti-tick grasses as the basis for developing practical tropical tick control packages. Trop Anim Health Prod 1978;10:179-182.

  6. Mwangi EN, Essuman S, Kaaya GP, Nyandat E, Munyinyin D, Kimondo MG. Repellence of the tick Rhipicephalus appendiculatus by the grass Melinis minutiflora. Trop Anim Health Prod 1995;27:211-216.

  7. Fernández-Ruvalcaba M, Cruz-Vázquez C, García-Vázquez Z, Saltigeral OJ. Estudio de seguimiento del efecto antigarrapata de las leguminosas tropicales Stylosanthes humilis (L.) y Stylosanthes hamata (L.) de un año de edad. Tec Pecu Mex 1999;37:51-56.

  8. Malonza MM, Dipelou OO, Amoo AO, Hassan SM. Laboratory and field observations on anti-tick properties of the plant Gynandropsis gynandra (L.) Brig. Vet Parasitol 1992;42:123-136.

  9. Pagano M, Gauvreau K. Principles of Biostatistics. 1st ed. New York:Duxbury Press, 1993.

  10. SAS. SAS/STAT users guide. Version 6.12. 4th ed. Cary (NC):SAS Institute Inc., 1989.

  11. Lwande W, Ndakala AJ, Hassanali A, Moreka L, Nyandat E, Ndungu M, et al. Gynandropsis gynandra essential oil and its constituents as tick (Rhipicephalus appendiculatus) repellents. Phytochemistry 1999;50:401-405.

  12. Flores MJ. Bromatología Animal. 3ª ed. México: Limusa, 1983.

  13. Humpreys LR. A guide to better pastures for the tropics and subtropics. Sidney:Wright Stepheson Co, 1980.

  14. Pegram RG, Tatchell RJ, de Castro JJ, Chizyuka HGB, Creek MJ, McCosker PJ, et al. Tick control: new concepts. World Anim Rev 1993;74-75:2-11.

  15. Kunz SE, Kemp DH. Insecticides and acaricides: Resistance and environmental impact. Rev Sci Tech 1994;13:1249-1286.

  16. Kaaya GP. Non chemical agents and factors capable of regulating tick populations in nature: a mini review. Insect Sci Appl 1992;13:587-594.

  17. Kaaya GP. The potential for anti-tick plants as component of an integrated tick control strategy. Ann NY Acad Sci 2000;916:576-582.

  18. Thompson KC, Roa E, Romero N. Anti-tick grasses as the basis for developing practical tropical tick control packages. Trop Anim Health Prod 1978;10:179-182.

  19. Mwangi EN, Essuman S, Kaaya GP, Nyandat E, Munyinyin D, Kimondo MG. Repellence of the tick Rhipicephalus appendiculatus by the grass Melinis minutiflora. Trop Anim Health Prod 1995;27:211-216.

  20. Fernández-Ruvalcaba M, Cruz-Vázquez C, García-Vázquez Z, Saltigeral OJ. Estudio de seguimiento del efecto antigarrapata de las leguminosas tropicales Stylosanthes humilis (L.) y Stylosanthes hamata (L.) de un año de edad. Tec Pecu Mex 1999;37:51-56.

  21. Malonza MM, Dipelou OO, Amoo AO, Hassan SM. Laboratory and field observations on anti-tick properties of the plant Gynandropsis gynandra (L.) Brig. Vet Parasitol 1992;42:123-136.

  22. Pagano M, Gauvreau K. Principles of Biostatistics. 1st ed. New York:Duxbury Press, 1993.

  23. SAS. SAS/STAT users guide. Version 6.12. 4th ed. Cary (NC):SAS Institute Inc., 1989.

  24. Lwande W, Ndakala AJ, Hassanali A, Moreka L, Nyandat E, Ndungu M, et al. Gynandropsis gynandra essential oil and its constituents as tick (Rhipicephalus appendiculatus) repellents. Phytochemistry 1999;50:401-405.

  25. Flores MJ. Bromatología Animal. 3ª ed. México: Limusa, 1983.

  26. Humpreys LR. A guide to better pastures for the tropics and subtropics. Sidney:Wright Stepheson Co, 1980.




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