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2016, Number 4

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Rev Odont Mex 2016; 20 (4)

Histological correlation of Ki-67 and D1 cycline markers expression in squamous cell carcinoma in the oral cavity

Acero-Mondragón E, Rodríguez-Farías R, Salazar-Hernández M, Figueroa-Avendaño J

Language: Spanish
References: 25
Page: 233-237
PDF size: 895.84 Kb.


ABSTRACT

Introduction: Oral squamous cell carcinoma (OSCC) is a malignant neoplasia originated in the epithelium’s keratocytes. Early biopsies reveal dysplastic mitotic keratocytes and sub-epithelial inflammatory infiltrate which progress towards a loss of basal membrane. Nevertheless, hematoxillin and eosin (H&E) histological diagnosis does not show suitable clinical correlation; this aspect improves when molecular markers such as Ki-67 and cyclin D1 are used. The aim of the present study was to correlate histological description of OSCC cases with Ki-676 and cyclin D1 expression. Material and methods: Twelve patients with lesions suggestive of oral cancer were analyzed, patients with OSCC diagnosis were included in the study. Single biopsies were taken, observing histological and technical processing for paraffin cuts, coloration with H&E and immunohistochemical coloration with cyclin D1 and Ki-67. Three patients met inclusion criteria, they were named C1, C2 and C3. OSCC type was classified according to the American Joint Committee on Cancer. Results: Histologically, C1 case was classified as type II OSCC. Cases C2 and C3 were classified as type I OSCC. Immunohistochemical analysis for C1 revealed Ki-67 positive and cyclin D1 negative; C2 exhibited Ki-67 negative and cyclin D1 positive, and C3 showed Ki-67 positive and cyclin D1 negative. Conclusions: Search for markers such as Ki-67 and cyclin D1 in pre-established OSCC diagnoses can influence histological results, contributing thus to more accurate diagnosis and treatment for the patients.


REFERENCES

  1. Barnes L, Eveson JW, Reichart P, Sidransky D. World Health Organization classification of tumours: pathology and genetics, head and neck tumours. Lyon: IARC Press; 2005. pp. 164-176.

  2. Zini A. Oral cancer over four decades: epidemiology, trends, histology, and survival by anatomical sites. J Oral Pathol Med. 2010; 39 (4): 299-305.

  3. Parkin DM, Whelan SL, Ferlay J, Teppo L, Thomas DB. Cancer incidence in five continents. Vol. VIII. Lyon: IARC Press; 2003.

  4. Tabaquismo. III Estudio Nacional de Salud Bucal ENSAB y V Estudio Nacional de Factores de Riesgo de Enfermedades Crónicas. ENFREC II. República de Colombia. Bogotá: Ministerio de Salud; 1999.

  5. Rodríguez E, Ronderos M. El hábito de fumar en Colombia 1977-1980. Bogotá: Organización Panamericana de la Salud, Ministerio de Salud, Instituto Nacional de Salud, Instituto Nacional de Cancerología; 1988.

  6. Álvarez ME, Barbosa CHM, Gaviria NAM. Comportamiento clínico-epidemiológico del carcinoma escamocelular bucal de pacientes tratados en el Hospital Universitario San Vicente de Paúl (HUSVP) Medellín entre enero de 1990 y diciembre de 1996. Rev Fac Odont Univ Ant. 2005; 16 (1 y 2): 26-32.

  7. Restrepo D, Correa C, Martínez C, Gómez G, Molina H. Frecuencia del consumo de bebidas alcohólicas entre los pacientes con cáncer bucal de la Unidad de Cirugía Maxilofacial y Estomatología del H.U.S.V.P., Medellín, entre enero de 1996 y junio de 2001. Rev Fac Odont Univ Ant. 2001; 13 (1): 58-64.

  8. Mashberg A, Samit A. Early diagnosis of asymptomatic oral and oropharyngeal squamous cancers. CA Cancer J Clin. 1995; 45: 328-351.

  9. Agra IM. Biological markers and prognosis in recurrent oral cancer after salvage surgery. Arch Otolaryngol Head Neck Surg. 2008; 134 (7): 743-749.

  10. de Jong EP, Xie H, Onsongo G, Stone MD, Chen XB, Kooren JA et al. Quantitative proteomics reveals myosin and actin as promising saliva biomarkers for distinguishing pre-malignant and malignant oral lesions. PLoS One. 2010; 5 (6): e11148.

  11. Chang YC. Invasive pattern grading score designed as an independent prognostic indicator in oral squamous cell carcinoma. Histopathology. 2010; 57 (2): 295-303.

  12. Odell EW, Jani P, Sherriff M, Ahluwalia SM, Hibbert J, Levison DA et al. The prognostic value of individual histologic grading parameters in small lingual squamous cell carcinomas. The importance of the pattern of invasion. Cancer. 1994; 74: 789-794.

  13. Weijers M. Malignancy grading is no better than conventional histopathological grading in small squamous cell carcinoma of tongue and floor of mouth: retrospective study in 128 patients. J Oral Pathol Med. 2009; 38 (4): 343-347.

  14. Goerkem M, Braun J, Stoeckli SJ. Evaluation of clinical and histomorphological parameters as potential predictors of occult metastases in sentinel lymph nodes of early squamous cell carcinoma of the oral cavity. Ann Surg Oncol. 2010; 17 (2): 527-535.

  15. Seki S. Prediction of outcome of patients with oral squamous cell carcinoma using vascular invasion and the strongly positive expression of vascular endothelial growth factors. Oral Oncol. 2011; 47 (7): 588-593.

  16. Shah NG. Prognostic significance of molecular markers in oral squamous cell carcinoma: a multivariate analysis. Head Neck. 2009; 31 (12): 1544-1556.

  17. Chimenos E, Font I, López J. Riesgo de cáncer oral y marcadores moleculares. Med Oral Patol Oral Cir Bucal. 2004; 9: 377-384.

  18. Wangsa D, Ryott M, Avall-Lundqvist E, Petersson F, Elmberger G, Luo J et al. Ki-67 expression predicts locoregional recurrence in stage I oral tongue carcinoma. Br J Cancer. 2008; 99 (7): 1121-1128.

  19. Mineta H, Miura K, Takebayashi S, Ueda Y, Misawa K, Harada H et al. Cyclin D1 overexpression correlates with poor prognosis in patients with tongue squamous cell carcinoma. Oral Oncol. 2000; 36 (2): 194-198.

  20. Vermeulen K, Van Bockstaele DR, Berneman ZN. The cell cycle: a review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif. 2003; 36 (3): 131-149.

  21. Montebugnoli L, Badiali G, Marchetti C, Cervellati F, Farnedi A, Foschini MP. Prognostic value of Ki67 from clinically and histologically normal distant mucosa in patients surgically treated for oral squamous cell carcinoma: a prospective study. Int J Oral Maxillofac Surg. 2009; 38 (11): 1165-1172.

  22. Chi AC. Epithelial pathology. In: Neville BW, Damm DD, Allen CM, Bouquot JE (Eds.). Oral and maxillofacial pathology. 3rd ed. St. Louis: Saunders, Elsevier; 2009. pp. 362-452.

  23. Kantola S, Jokinen K, Hyrynkangas K, Mäntyselkä P, Alho OP. Detection of tongue cancer in primary care. Br J Gen Pract. 2001; 51 (463): 106-111.

  24. Saiz-Bustillo R, Corchero MG, García-Montesinos PB, González TT, Sánchez SS. Carcinoma oral de células escamosas: parámetros citométricos de interés pronóstico. Med Oral patol Oral Cir Bucal. 2005; 10 (5): 462-467.

  25. Huang SF, Cheng SD, Chuang WY, Chen IH, Liao CT, Wang HM et al. Cyclin D1 overexpression and poor clinical outcomes in Taiwanese oral cavity squamous cell carcinoma. World J Surg Oncol. 2012; 10: 40.




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