Kusznierz G, Chiani Y, Rudi J, Ortellao L, Cantarutti D, Pierini J, Cociglio R, Sioli N, Ricart M, Solari V, Carreño G, Vera GV, Gasparri J

Language: Spanish
References: 20
Page: 323-334
PDF size: 163.20 Kb.

ABSTRACT

Introduction: Streptococcus pneumoniae is the main etiologic agent for communityacquired pneumonia.
Objective: to test plasma, whole blood, white blood cells and serum samples in order to detect Streptococcus pneumoniae deoxyribonucleic acid.
Methods: nested polymerase chain reaction technique detected pneumolysin gen fragments in several blood components from patients with community-acquired pneumonia.
Results: polymerase chain reaction was positive in 69 (27.7 %) of 249 patients with community-acquired pneumonia. Whole blood was positive in 36 patients (14.4 %; 95 % CI, 10-19 %) plasma in 31 (12.4 %; 95 % CI, 8-16 %), buffy coat in 29 (11.6 %; 95 % CI, 8-16 %) and serum in 22 (8.8 %; 95% CI, 5-12 %). Positivity increased with greater use of three or four blood components and statistically significant differences were found. By using a single sample (whole blood), 48 % of confirmed cases would have been undetected whereas the serum sample would have just diagnosed 30 % of cases.
Conclusions: this study proves that diagnosis of invasive pneumococcal infection in children suffering community-acquired pneumonia requires a combined use of several blood components. The four blood fractions are vital to attain optimal susceptibility and to make definitive diagnosis of all the cases.

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