medigraphic.com

2015, Number 2

<< Back Next >>

Cuba y Salud 2015; 10 (2)

Microbiological characterization of Staphylococcus aureus isolations in purulent samples. “Fructuoso Rodríguez” Orthopedic Hospital. January to May, 2014

Duquesne AA, Castro SN, Monzote LA, Paredes CI

Language: Spanish
References: 25
Page: 17-22
PDF size: 844.28 Kb.


ABSTRACT

Objective: To characterize the isolations of Staphylococcus aureus recuperated from the purulent samples of patients with infections associatedwithsanitary and community.
Method: Transverse descriptive study conducted at “Fructuoso Rodríguez” Orthopedic Hospital from January to May 2014. 242 purulent samples were analyzed, 134 obtained from surgical wounds infected of hospitalized patients and 108 from skin infections and soft parts of the community cases. The samples process was developed through microbiological conventional methods; antimicrobial susceptibility was determined in those where S. aureus was recuperated by Bauer-Kirby and E-test methods for the vancomycin.
Results: S. aureus was identified in 41,0 y 50,9% of the hospital and communitarian isolations respectively ; 52,7% of those performed in the hospital was obtained from infected surgical wounds and 94,6% from infections of the skin and soft parts. S. aureus proved resistance to methicillin (SARM) in 50,9 and 25,5% in the isolations worked out in hospital and in the community respectively. Strains resistant to vancomycin were not observed.
Conclusions: S. aureus predominates in the purulent samples obtained (hospital and community) according to the international and national literature consulted. Cotrimoxazol showed a higher percentage of resistance in the community, what presumesits irrational use in the community as a possible cause of this phenomenon being concerned.


REFERENCES

  1. Harbarth S, Fankhauser C, Schrenzel J, Christenson J, Gervaz P, Bandiera–Clerc C, Renzi G, Vernaz N, Sax H, Pittet D. Universal screening for methicillin–resistant Staphylococcus aureus at hospital admission and nosocomial infection in surgical patients. The Journal of the American Medical Association. 2008; 299:1149–1157.

  2. Schramm G, Johnson J, Doherty J, Micek S, Kollef M. Methicillin–resistant Staphylococcus aureus sterile–site infection: the importance of appropriate initial antimicrobial treatment. Critical Care Medicine. 2006; 34:2069–2074.

  3. Talbot GH, Bradley J, Edwards JE, Gilbert D, Scheld M, Bartlett JG. Bad bugs needs drugs: an update on the development pipeline from the antimicrobial availability task force. Clin Inf Dis. 2006; 42:657-68.

  4. National Nosocomial Infectious Surveillance System (NNIS). System Report, data summary from January 1992 through June 2004, issued October 2004. Am J Infect Control. 2004; 32: 470-85.

  5. Diederen BM, Kluytmans JA. The emergence of infections with community-associated methicillin resistant Staphylococcus aureus. J Infect. 2006; 52:157-68.

  6. Sociedad Española de Medicina Preventiva, Salud Pública e Higiene. 2011. Estudio de prevalencia de las infecciones nosocomiales en España. EPINE 1990-2011: 22 años. 22º Estudio. Informe Global de España [monografía en Internet]; España [citado 8 de febrero de 2012]. Disponible en: http://www.vhebron.net/ac/preventiva/epine/9-epine_1990-2011.pdf

  7. Ruiz Bremon A, Ruiz Tovar M, Perez Gorricho B, Díaz de Torres P, López Rodríguez R. Non-hospital consumption of antibiotics in Spain: 1987-1997. J Antimicrob Chemother. 2000; 45: 395-400.

  8. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 20th informational supplement (M105- S19). Wayne, Pa. USA: Clinical and Laboratory Standards Institute, 2014.

  9. Sobhya N, Alyb F, Abd O, Ghazalb A, Elbaradeic A. Community-acquired methicillin-resistant Staphylococcus aureus from skin and soft tissue infections (in a sample of Egyptian population): analysis of mec gene and staphylococcal cassette chromosome. braz j infect dis. 2012;16:426–431.

  10. Pofahl W, Ramsey K, Nobles D, Cochran M, Goettler C. Importance of Mehicillin–Resistant Staphylococcus aureus eradication in carriers to prevent postoperative methicillin–resistant Staphylococcus aureus surgical site infection. The American Surgeon. 2011; 77: 27–31.

  11. Lambert M, Suetens C, Savey A, Palomar M, Hiesmayr M, Morales I, Agody A, Frank U, Mertens K, Schumacher M, Wolkewitz M. Clinical outcomes of health–care–associated infections and antimicrobial resistance in patients admitted to European intensive– care units: a cohort study. The Lancet Infectious Diseases. 2011; 11: 30–38.

  12. Centers for Disease Control and Prevention (CDC). Methicillin-resistant Staphylococcus aureus infections among competitive sports participants — Colorado, Indiana, Pennsylvania, and Los Angeles County, 2000-2003. MMWR Morb Mortal Wkly Rep. 2003; 52:793–5.

  13. Mederos J, Morejón M. Frecuencia de aislamientos de Staphylococcus aureus resistente a meticillina en el Hospital “Manuel Fajardo Rivero” Revista Habanera de Ciencias Médicas. 2014;13:406-416.

  14. Cabrera Rodríguez LE, Díaz Rigau L, Fernández Núñez T, Bravo Fariñas L. Susceptibilidad antimicrobiana de aislamientos bacterianos causantes de infecciones comunitarias Rev Cubana Med Gen Integr. 2007; 23: 1-5.

  15. Morejón M. Staphylococcus aureus resistente a meticillina. Un problema actual. Dermatol Perú. 2012;22:29-33.

  16. Moran GJ, Krishnadasan A, Gorwitz RJ. Methicillin resistant S. aureus infections among patients in the emergency department. New Engl J Med.2006; 355:666–674.

  17. Dufour P, Gillet Y, Bes M. Community-acquired methicillin-resistant Staphylococcus aureus infections in France: emergence of a single clone that produces Panton–Valentine leukocidin. Clin Infect Dis. 2002; 35:819–824.

  18. Rossi F, García P, Ronzon B. Rates of antimicrobial resistance in Latin America (2004-2007) and in vitro activity of the glycylcycline tigecycline and of other antibiotics. Braz J Infect Dis. 2008; 12: 405-15.

  19. Acosta Pérez G, Rodríguez Abrego G, Longoria Revilla E, Castro Mussot ME. Evaluación de cuatro métodos para la detección de Staphylococcus aureus meticilino-resistente de muestras clínicas en un hospital regional. Salud Publica Mex. 2012; 54:1-6.

  20. Nodarse R. Detección de Staphylococcus aureus resistente a meticillina mediante disco de cefoxitina. Revista Cubana de Medicina Militar. 2009; 38:30- 9.

  21. Latin American Resistance Surveillance Network. Annual report on the antibiotic resistance monitoring/surveillance network. Ginebra: 2008.

  22. Mithoe D, Rijnders J. Prevalence of community-associated meticillin-resistant Staphylococcus aureus and Panton–Valentine leucocidin positive S. aureus in general practice patients with skin and soft tissue infections in the northern and southern regions of The Netherlands. Eur J Clin Microbiol Infect Dis. 2012; 31:349–356.

  23. Kaplan SL. Clinical implications of community-acquired methicillin-resistant Staphylococcus aureus. Program and abstracts of the Pediatrics Academic Societies Annual Meeting; May 14-17, 2005. Washington, DC. Course # 5102.

  24. Naimi TS, LeDell KH, Como-Sabetti K, et al. Comparison of community- and health care-associated methicillin resistant Staphylococcus aureus infection. JAMA. 2003; 290: 2976–84.

  25. Prego J, Galiana A, Pujadas M, Almada K. Infecciones de piel y partes blandas en pacientes ambulatorios. Rev Chil Pediatr. 2006; 77:196-197.




2020     |     www.medigraphic.com