Espinoza-Santillán D, Martínez-Juárez V, Peralta-Ortiz J, Molina- Mendoza P, Olave-Leyva J, Ávila-Castillo R

Language: Spanish
References: 15
Page: 22-28
PDF size: 64.43 Kb.

ABSTRACT

The aim of this study was to evaluate the presence and identification of bacterial microorganisms in uterus from slaughtered cows. Thirty-two samples of uterine lavage were obtained, these samples were used to inoculate blood agar and McConkey agar culture media, subsequently these underwent Gram staining for classification in Gram- positive and Gram-negative. A Catalase test, besides a mannitol salt agar culture and coagulase, were performed to the Gram-positive bacteria, while to the Gram-negative bacteria, the IMViC tests were performed. 100% of the samples showed bacterial isolation; from these, 40.62% were coagulase-negative Staphylococcus (SCN), likely epidermis species, representing the highest frequency of bacterial isolates (P ‹0.05). Among the Gram-negative isolates Salmonella spp. prevailed with 15.62% and 12.5% for Escherichia coli. The results demonstrate that, in the uterus from slaughtered cows at the slaughterhouse, bacterial organisms, live as part of normal microbiota of the uterus, however, these bacteria are also described as opportunistic pathogens that cause different degrees of uterine infection, which may be related with reproductive tract infections, and adverse effects on fertility, encouraging the animal sacrifice.

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