2016, Number 2
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Rev Biomed 2016; 27 (2)
Detection of hepatitis E virus genotype 1d in patients with suspect of acute viral hepatitis in cuba during 2013
Montalvo-Villalba MC, Castellano- Girones Y, Bello-Corredor M, Rodríguez-Lay LÁ
Language: Spanish
References: 40
Page: 75-83
PDF size: 288.77 Kb.
ABSTRACT
Introduction. Hepatitis E virus (HEV) is mainly transmitted by the fecal-oral route and is one of the most important causes of acute viral hepatitis (AVH) around the world. In Cuba, Despite of endemic behavior of HEV in Cuba, its causality is not associated when a picture of enteric acute hepatitis is suspected.
Objective. Taking into account the common transmission route of both HEV and HAV, our aim
was to estimate the incidence of HEV infection in sera samples received throughout the country during 2013 where the IgM anti-HAV test was required by the Clinician.
Materials and Methods. A specific RT-PCR for open reading frame 2 (ORF2) was used to detect RNA-HEV in 422 sera. The amplified products corresponding to ORF2 were purified, sequenced and phylogenetically analyzed using MEGA6 software program.
Results. RNA-HEV was detected in 8.53% (36/422) of the samples. The highest rate of positivity was identified in the Western region of the country, specifically in Havana 5.45% (23/422). IgM anti-HAV was detected in 5.21% (22/422) and simultaneous detection of both HAV and HEV was found in 13.88% (5/36) of the samples. The results showed a higher incidence of HEV with respect to HAV (8.53% vs 5.21%) and phylogenetic analysis showed the circulation of genotype 1, subgenotype 1d of the HEV.
Conclusions. This study corroborated the endemicity of HEV and for the first time the subgenotype 1d, the African variant strain associated to outbreak of hepatitis E, is reported in viral hepatitis cases in Cuba.
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