León MG, Osorio FMR, Martínez USR

Language: Spanish
References: 21
Page: 742-750
PDF size: 102.63 Kb.

ABSTRACT

Objectives: to assess the chemical composition and physicochemical properties of essential oil of the plant species Citrus sinensis L, obtained by hydrodistillation and microwave radiation-assisted hydrodistillation, and to make a comparison between two extraction methods used.
Methods: essential oils were extracted by distillation and microwave radiationassisted hydrodistillation from the fruit pericarp; relative density at 20°C, refractive index and the essential oils solubility in ethanol (70% v/v) were determined. The chemical composition was assessed using chromatography mass spectrometer/gas.
Results: the yields ranged from 0,51 to 0,42%, depending on the extraction method used. The essential oil of Citrus sinensis L. by both methods had limonene as a major component. A higher content of essential oil was obtained when the microwave radiation-assisted method was used compared with the conventional hydrodistillation.
Conclusions: the yield of the essential oil from Citrus sinensis L. (Orange) depends on method of extraction used; microwave-assisted hydrodistillation is faster and more efficient method than the conventional hydrodistillation.

REFERENCES

1. Matiz G, Osorio MR, Camacho F, Atencia M, Herazo J. Diseño y evaluación in vivo de fórmulas para acné basadas en aceites esenciales de naranja (Citrus sinensis), albahaca (OcimumbasilicumL) y ácido acético. Biomédica: Revista del Instituto Nacional de Salud. 2012;32(1):1-27.

2. Juárez JR, Castro AJ, Jaúregui JF, Lizano JV, Carhuapoma M, Choquesillo FF, et al. Chemical composition, antibacterial activity of essential oil Citrus sinensis L. (Sweet orange) and formulation of a pharmaceutical form.Ciencia e Investigación. 2010;13(1):9-13.

3. Sulbarán B, Ojeda G, Ysambertt F, Cabrera L. Volatile fraction composition of Venezuelan sweet orange essential oil (Citrus sinensis). Ciencia. 2003;11(1):55-60.

4. Martínez J, Sulbarán B, Ojeda G, Ferrer A, Nava R. Actividad antibacteriana del aceite esencial de mandarina. Rev. Fac. Agron. 2003; 20:502-512.

5. Olivero J, Caballero K, Jaramillo B, Stashenko EE. Repellent activity of the essential oils from Lippiaoriganoides, Citrus sinensis and Cymbopogonnardus cultivated in Colombia against Triboliumcastaneum, Herbst. Salud UIS. 2009;41:244-250.

6. Vásquez O, Alva A, Marreros J. Extracción y caracterización del aceite esencial de Jengibre (Zingiberofficinale). Revista Amazónica de Investigación Alimentaria. 2001;1(1):38–42.

7. Guerra L, Soto L, Medina Z, Ojeda G, Peña J. Antibacterial activity of the essential oil of orange barks (Citrus sinensis) var. Valencia against positive and negative gram microorganisms. Rev. Fac. Agron. (LUZ). 2014;31:215-232.

8. Lota ML, de Rocca Serra D, Jacquemond C, Tomi F, Casanova J. Chemical variability of peel and leaf essential oils of sour orange. FlavourFragr. J. 2001;16:89–96.

9. Golmakani M, Rezaei K. Comparison of microwave-assisted hydrodistillation with the traditional hydrodistillation method in the extraction of essential oils from Thymus vulgaris L. Food Chemistry. 2008;109(4):925-930.

10. Chemat F, Lucchesi ME, Smadja J, Favretto L, Colnaghi G, Visinoni F. Microwave accelerated steam distillation of essential oil from lavender: A rapid, clean and environmentally friendly approach. Analytica Chimica Acta. 2006;55(1):157-160.

11. Rincón CA, Castaño JC, Ríos E. Actividad biológica de los aceites esenciales de Acmella ciliata (Kunth) Cass. Revista Cubana de Plantas Medicinales. 2012;17(2):160-171.

12. Torrenegra M, Matiz G, León G. Actividad antibacteriana in vitro de aceites esenciales frente a microorganismos implicados en el acné. Revista Cubana de Farmacia. 2015 [Citado: 12 de noviembre de 2015]. 49;(3). Disponible en: http://bvs.sld.cu/revistas/far/vol49_3_15/far11315.htm

13. Tomy GT, Stern GA, Muir DC, Fisk AT, Cymbalisty CD, Westmore JB. Quantifying C10-C13 polychloroalkanes in environmental samples by highresolution gas chromatography/electron capture negative ion high-resolution mass spectrometry. Anal Chem. 1997;69(14):2762-71.

14. Baharum SN, Bunawan H, GhaniMaA, Mustapha WAW, Noor NM. Analysis of the chemical composition of the essential oil of Polygonum minus Huds.using twodimensional gas chromatography-time-of-flight mass spectrometry (GC-TOF MS). Molecules. 2010;15(10):7006-15.

15. Torrenegra M, Granados C, Osorio M, León G. Method comparison of hydrodistillation microwave radiation-assisted (MWHD) front hydrodistillation (HD) in the extraction of essential oil of Minthostachys mollis. Inf. Tecnol. 2015;26(1):117-122.

16. Stashenko E, Ruíz CA, Arias G, Durán DC, Salgar W, Cala M, Martínez MR. Lippia origanoides chemotype differentiation based on essential oil GC-MS analysis and PCA. J. Sep. Sci. 2010;33:93-103.

17. Yáñez X, Lugo LL, Parada DY. Estudio del aceite esencial de la cáscara de la naranja dulce (Citrus sinensis, variedad Valenciana) cultivada en Labateca (Norte de Santander, Colombia). BISTUA. 2007;5(1):3-8.

18. Granados C, Yáñez Y, Santafé G. evaluación de la actividad antioxidante del aceite esencial foliar de Calycolpus moritzianus y Minthostachys mollis de Norte de Santander. Bistua: Revista de la Facultad de Ciencias Básicas. 2012;10(1):12-23.

19. Tafurt G, Martinez J, Stashenko E. Evaluación de la actividad antioxidante de aceites esenciales en emulsiones degradadas por radiación ultravioleta. Revista Colombiana de Química. 2005;34(1):43-55.

20. Wang HW, Liu YQ, Wei SL, Yan ZJ, Lu K. Comparison of Microwave-Assisted and Conventional Hydrodistillation in the Extraction of Essential Oils from Mango (Mangifera indica L.) Flowers. Molecules.2010;15,7715-23.

21. Wang HW, Liu YQ, Wei SL, Yan ZJ, Jin X.Comparative Chemical Composition of the Essential Oils Obtained by Microwave-Assisted Hydrodistillation and Hydrodistillation from Agrimonia pilosa Ledeb. Collected in Three Different Regions of China. Chemistry & Biodiversity. 2012;9(3):662–668.