Leite VI, de Morais OCD, Gomes FJ, Maia FPA, Relison S, Ferreira MEF, Alexandre APE, Melo CHD, de Alencar FJV, Ferreira LL, Gomes FF

Language: Portugués
References: 67
Page: 719-733
PDF size: 267.80 Kb.

ABSTRACT

Introduction: the study of medicinal plants has been used for medical and pharmaceutical services, but mainly used as an alternative or in addition to therapeutic treatment.
Objective: this study aimed to elucidate the main secondary metabolites and antioxidant activity and to evaluate the antifungal and modulating activity of the ethanol extract from Anadenanthera macrocarpa leaves against genus Candida strains.
Methods: the chemical component clearance was made by using high-performance liquid chromatography, and checking of the antioxidant activity was performed by FRAP method. The antifungal activity of the extract was measured by determining the minimum inhibitory concentration, the modulation performed by microdilution test, carried out to verify the interactions between the natural product and the antifungal product using the sample at a subinhibitory concentration.
Results: high-performance liquid chromatography showed the presence of secondary metabolites, such as: quercitrin, quercetin, isoquercitrin, apigenin, caffeic acid and gallic acid. In the FRAP assay, the reduction was 2,72 mg per equivalence grams of FeSO4 extract. The results of the assessment of minimum inhibitory concentration were ≥ 512 µg/mL and in the evaluation of tests for modulating effect, the extract ethanol did not potentiate the antifungal effect, with neither synergism nor antagonism.
Conclusions: from the results we can conclude that this extract can be an alternative since it has chemical constituents with known biological activities and antioxidants with low toxic potential.

REFERENCES

1. Novais TS, Costa JFO, David JPL, David JM, Queiroz LP, França F, et al. Atividade antibacteriana em alguns extratos de vegetais do semi-árido brasileiro. Rev Bras Farmacog. 2003;13:5-8.

2. Macedo M, Carvalho JMK, Nogueira FL. Plantas medicinais e ornamentais da área de aproveitamento múltiplo de Manso, Chapada dos Guimarães, Mato Grosso. Cuiabá: Editora da UFMT; 2002. p.188.

3. Simões CMO, Schenkel EP, Gosmann G, Mello JCP de, Mentz LA, Petrovick PR. Farmacognosia: da planta ao medicamento. 5. ed. Porto Alegre/Florianópolis: Editora da UFSC; 2004. 1102p.

4. Albuquerque U, Hanazaki N. As pesquisas etnodirigidas na descoberta de novos fármacos de interesse médico e farmacêutico: fragilidades e perspectivas. Rev Bras Farmacog. 2006;16:678-689.

5. Pereira RJ & Cardoso MC. Metabólitos secundários vegetais e benefícios antioxidantes Vegetable secondary metabolites andantioxidants benefits. J Biotec Biodivers. 2012;3(4):146-152.

6. Alves PM, Queiroz LMG, Pereira JV, Pereira MSV. Atividade antimicrobiana, antiaderente e antifúngica in vitro de plantas medicinais brasileiras sobre microrganismos do biofilme dental e cepas do gênero Candida. Rev Soc Bras Med Trop. 2009;42:222-224.

7. Volpato AMM. Avaliação do potencial antibacteriano de Calendula officinalis (Astearaceae) para seu emprego como fitoterápico. [Dissertação de doutorado]. Paraná: Universidade Estadual de Ponta Grossa; 2005. P.58-67.

8. Kauffman CA. Fungal infections: Proceedings of the American. Thoracic Society.2006;3:35-40.

9. Rossi T, Lozovoy MAB, Silva RV, Fernandes EV, Geraldino TH, Costa IC. Interações entre Candida albicanse hospedeiro. Semina: Ciências Biológicas e da Saúde. 2011;32:15-28.

10. Matos FJA. Introdução a fitoquímica experimental. Fortaleza: UFC Edições; 1997. p. 141.

11. Dantas JP, Benvindo FS, Sousa JH, Almeida JM, Figueiredo MC, Pequeno AS, et al. Avaliação da atividade antimicrobiana in vitro e determinação da concentração inibitória mínima (CIM) de extratos hidroalcoolico de angico sobre cepas de Staphylococcusaureu. RevBras Anal Clin. 2010;42:33-37.

12. Silva OK. Avaliação das atividades antimicrobiana, aderência, antioxidante, antiinflamatória e antinociceptiva de Anadenantheramacrocarpa (Benth) Brenan. [Dissertação Mestrado]. Bahia: Universidade Federal da Bahia; 2011. p.67-74.

13. Barbosa-Filho JM, Nascimento Júnior FA, Tomaz ACA, Athayde-Filho PF, Silva MS, Cunha EVL, et al. Natural products with antileprotic activity. Rev Bras Farmacog. 2007;17:141-8.

14. Brasileiro BG, Pizziolo VR, Raslan DS, Jamal CM, Silveira D. Antimicrobial and cytotoxic activities screening of some Brazilian medicinal plants used in Governador Valadares district, Rev Bras Cienc Farm. 2006;19:195-202.

15. Silva ARH, Moreira LR, Brum ES, Freitas ML, Boligon AA, Athayde ML, et al. Biochemical and hematological effects of acute and sub-acute administration to ethyl acetate fraction from the stem bark Scutia buxifolia Reissek in mice. J Ethnopharmacol. 2014;53:908-916.

16. Boligon AA, Kubiça TF, Mario DN, Brum TF, Piana M, Weiblen R, et al. Antimicrobial and antiviral activity-guided fractionation from Scutiabuxi folia Reissek extracts. Acta Physiol Plant. 2013;35:2229-2239.

17. Benzie IEF & Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of-antioxidant power: the FRAP assay. Anal Biochemistry. 1999;239:0-76.

18. ClinicalandLaboratory Standards Institute-CLSI. Os padrões de desempenho para testes de susceptibilidade antimicrobiana; 22. Suplemento informativo. M100- S22 documento. ClinicalLaboratory Standards Institute, Wayne, PA; 2012.

19. Coutinho HDM, Costa JG, Lima EO, Falcão-Silva VS, Siqueira-Júnior JP. Enhancement of the antibiotic activity against a multiresistant Escherichia coli by Menthaarvensis L. and chlorpromazine.Chemotherapy. 2008;54:328-330.

20. Matias EFF, Alves EF, Santos BS, Souza CES, Ferreira JVA, Lavor AKLS, et al. Biological Activities and Chemical Characterization of Cordiaverbenacea DC. as Tool to Validate the Ethnobiological Usage. J Evid Based Complementary Altern Med. 2013:1-7.

21. Penna C, Marino S, Vivot E, Cruanes MC, Munoz JD, Cruanes J, et al. Antimicrobial activity of Argentine plants used in the treatment of infectious diseases. Isolation of active compounds from Sebastiania brasiliensis. J Ethnopharmacol. 2000;77:37-40.

22. Peng ZF, Strack D, Baumert A, Subramaniam R, Goh KN, Chia TF, et al. Antioxidant flavonoids from leaves of PolygonumhydropiperL. Phytochemistry. 2003;62:219-228.

23. Novoa BE, Céspedes AC, De Garcia LA, Olarte CJE. Quercitrin, a flavonoid with hypotensive activity obtained from Croton glabellus. Rev Colomb Cienc Quim Farm.1985; 4:4-17.

24. Kang TH, Jeong SJ, Kim NY, Higuchi R, Kim YC. Sedative activity of two flavonol glycosides isolated from the flowers of Albizzia julibrissin Durazz. J Ethnopharmacol. 2000;71:321-323.

25. Fermin SMLH, Galvez J, Romero JA, Zarnuelo A. Effect of quercitrin on acute and chronic experimental. J PharmacolExpTher. 1996;278:771-779.

26. Comalada M, Camuesco D, Sierra A, Balleste I, Xaus J, Galvez J, et al. In vivoquercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-jB pathway. Eur J Immunol. 2005;35:584-592.

27. Musci I, Gyulai Z, Beladi I. Combined effects of flavonoids and acyclovir against herpes viruses in cell cultures. Acta Microbiol Immunol Hung. 1992;39:137-147.

28. Silva MC, Yunes RA, DelleMonache F, Santos ARS, Schmeling LO, Gadotti VM, et al. Phytochemical and pharmacological analysis ofBauhinia microstachya (Raddi) Macbr. (Leguminosae). Z N aturforsch C. 2001;56:939-942.

29. Czinner E, Hagymas IK, Blazovics A, Kery A, Szoke E, Lemberkovics E. The in vitro effect of Helichrysiflos on microsomal lipid peroxidation. J Ethnopharmacol. 2001;77:31-35.

30. Veras HNH, Santos IJM, Santos ACB, Fernandes CN, Matias EFF, Leite GO, et al. Comparative evaluation of antibiotic and antibiotic modifying activity of quercetin and isoquercetin in vitro. Curr Top Nutraceut R. 2011;9:25-29.

31. Vitor RF, Mota-Felipe H, Teixeira G, Borges C, Rodrigues AI, Teixeira AP. Flavonoids of an extract of Pterospartumtridentatum showing endothelial protection against oxidative injury. J Ethnopharmacol. 2004;93:363-370.

32. Kim HY, Moon BH, Lee HJ, Choi DH. Flavonol glycosides from the leaves of Eucommiaulmoides glycation anhibitory activity. J Ethnopharmacol. 2004; 93:227-230.

33. Calzada F, Cedillo-Rivera R, Mata R. Antiprotozoal activity of the constituents of Conyzafilaginoides. J Nat Products. 2001;65(5):671-673.

34. Krogh R, Kroth R, Berti C, Madeira AO, Souza MM, Cechinel-Filho V, et al. Isolation and identification of compounds with antinociceptive action from Ipomoea pes-caprae (L.) R. Br. Pharmazie. 1999;54:464-466.

35. Silva MC. Análise fitoquímica e farmacológica de plantas medicinais selecionadas da flora catarinense: Aleuritesmoluccana, Bauhiniamicrostachya, e Marrubiumvulgare. [Tese de Doutorado]. Santa Catarina: Universidade Federal de Santa Catarina; 2003. p. 128-138.

36. Wan L, Guo C, Yu Q, Li Y, Wang X, Wang X, et al. Quantitative determination of apigenin and its metabolism in rat plasma after intravenous bolus administration by HPLC coupled with tandem mass spectrometry. J Chromatogr B. 2007;855:286-289.

37. Svehlíková V, Bennett RN, Mellon FA, Needs PW, Piacente S, Kroon PA, et al. Isolation, identification and stability of acylatedderivates of apigenin 7-O-glucoside from chamomile (Chamomillarecutita L. Rauschert.). Phytochemistry, 2004;65:2323-2332.

38. Schulz V, Hansel R, Tyler VE. Fitoterapia Racional: Um guia de Fitoterapia para as Ciências da Saúde. 4. ed. Barueri: Editora Manole; 2002. p. 307-311.

39. Zekovic Z, Pekic B, Lepojevic Z, Petrovic L. Chromatography in our investigations of Camomile (Matricariachamomilla L.). J Chromatogr B. 1994;39:9-10.

40. Soares SE. Ácidos fenólicos como antioxidantes. Rev Nutr. 2002;15:71-81.

41. Morais-Braga MFB. Prospecção química do extrato etanólico das folhas de lygodiumvenustumsw (Lygodiaceae) e avaliação das bioatividades antioxidante, antiepimastigota, antipromastigota, citotóxica e antimicrobiana de extrato e frações ( in vitro). [Dissertação de Mestrado]. Ceará: Universidade Regional do Cariri; 2012. p. 165-170.

42. Krogh R, Tunes RA, Andricopulo AD. Structure-activity relationships for the analgesic activity of gallic acid derivates. Il Farmaco. 2001;55(11-12):730-735.

43. Inoue M, Suzuki R, Koide T, Sakaguchi N, Ogihara Y, Yabu Y. Antioxidant, gallic acid, induces apoptosis in HL-60RG cells. BiochemBiophys Res Commun. 1994;204:898-904.

44. Figueredo FG, Ferreira EO, Lucena BFF, Torres CMG, Lucetti DL, Lucetti ECP, et al. Modulation of the Antibiotic Activity by Extracts from Amburanacearensis A. C. Smith and Anadenan theramacrocarpa (Benth.) Brenan. Biomed Res Int. 2013;1:1-5.

45. Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacology & Therapeutics. 2002;96:67-202.

46. Walle T. Flavonoids and isoflavones (phytoestrogens): absorption, metabolism and bioactivity.Free Radical Bio Med. 2004;36:829-837.

47. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Anti-inflammatory properties of plant flavonoids: effects of rutin, quercetin and hesperedin on adjuvant arthritis in rat. Fármaco. 2001;56:683-687.

48. Narayana KR, Reddy MS, Chaluvadi MR, Krishna DR. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potencial. Indian J Pharmacol. 2001;33:2-16.

49. Harborne JB & Williams CA. Advances in flavonoids research since 1992. Phytochemistry. 2000;55:481-504.

50. Di Carlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci. 1999;65:337-353.

51. Formica LV, Regelson W. Review of the biology of quercetin and related bioflavonoids. Food Chem Toxicol. 1995;33:1061-1080.

52. Uchôa VT, Carvalho AA, Sousa CMM, Chaves MH, Sant’ana AEG. Evaluation of the antioxidizing activity of isolated Ximeniaamericana L. extracts and compounds. In: 1ST Brazilian Conference on Narural Products and XXVII Annual Meeting on Micromolecular Evolution, Systematics and Ecology [RESEM]; São Pedro, SP; 2007.

53. Jomova K, Vondrakova D, Lawson M, Valko M. Metals, oxidative stress and neurodegenerative disorders. Mol Cell Biochem. 2010;345:91-104.

54. Jomova K, Jenisova Z, Feszterova M, Baros S, Liska J, Hudecova D, et al. Arsenic: Toxicity, oxidative stress and human disease. J Appl Toxicol. 2011;31:95-107.

55. Andrade CA, Costa CK, Bora K, Miguel MD, Miguel OG, Kerber VA. Determinação do conteúdo fenólico e avaliação da atividade antioxidante de Acaciapodalyriifolia A. Cunn. ex G. Don, Leguminosae-mimosoideae. Rev Bras Farmacogn. 2007;17:231-235.

56. Ajaikumar KB, Asheef M, Babu BH, Padikkala J. The inhibition of gastric mucosal injury by Punicagranatum L. (pomegranate) methanolic extract. J Ethnopharmacol. 2005;96(1):171-176.

57. Stratil P, Klejdus B, Kuban V. Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods. J Agr Food Chem. 2006;54(3):607-616.

58. Hukkanen AT, Pölönen SS, Kärenlampi SO, Kokko HI. Antioxidant capacity and phenolic content of sweet rowanberries. J AgrFoodChem. 2006;54(1):112-119.

59. Sousa CMM, Silva HR, Vieira-Junior GM, Ayres MCC, Costa CLS, Araújo DS, et al. Fenóis totais e atividade antioxidante de cinco plantas medicinais. Quim Nova. 2007;30(2):351-355.

60. Saxena R, Venkaiah K, Anitha P, Venu L, Raghunath M. Antioxidant activity of commonly consumed plant foods of India: contribution of their phenolic content. Int J Food SciNutri. 2007;58(4):250-260.

61. Singh RP, Chidambara Murthy KN, Jayaprakasha GK. Studies on the Antioxidant Activity of Pomegranate (Punicagranatum) Peel and Seed Extracts Using in vitro Models.J Agr Food Chem. 2002;50:81-86.

62. DesmarchelierC, Lisboa Romão R, Coussio J, Ciccia G. Atividades de eliminação de radicais livres em antioxidantes e extratos de plantas medicinais utilizadas na região de caatinga no Nordeste do Brasil.J Etnofarmacol. 1999;67(1):69-77.

63. Karakaya S. Bioavailability of phenolic compounds. Crit Rev Food Sci Nutr. 2004;44(6):453-464.

64. Costa EMMB, Barbosa AS, Florentino VGB, Silva JDF, Trovão DMBM, Medeiros ACD. In vitro antimicrobial activity of plant extracts of semi-arid region of Paraíba, PB, Brazil. Rev Odonto Cienc. 2013;28(4):101-104.

65. Lima RF, Alves EP, Rosalen PL, Ruiz ALTG, Duarte MCT, Góes VFF, et al. Antimicrobial and Antiproliferative Potential of Anadenantheracolubrina(Vell.) Brenan. J Evid Based Complementary Altern Med.2014;2014:1-7.

66. Carvalho AVOR. Atividade antimicrobiana in vitro de plantas do semiárido paraibano sobre espécies de Streptococcus e Cândida TCC. [Dissertação de Mestrado]. Paraíba: Universidade Estadual da Paraíba; 2012. p. 36-41.

67. Weber-sobrinho CR. Determinação da atividade antimicrobiana e citotóxica de extratos e da casca do caule de anadenanthera colubrina (Vell.) Brenan var. Cebil(Griseb.) Von Reis Alt. (Angico de Caroço). [Dissertação de Mestrado]. Pernambuco: Universidade Federal de Pernambuco; 2010. p. 154-160.