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ISSN 1561-2988 (Print)

2015, Number 3

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Rev Cubana Farm 2015; 49 (3)

In vitro antibacterial activity of the essential oil against microorganisms involved in acne

Torrenegra AME, Matiz MGE, Gil GJ, León MG

Language: Spanish
References: 23
Page: 512-523
PDF size: 178.63 Kb.


ABSTRACT

Objective: to assess the chemical composition, antibacterial sensitivity and the in vitro minimum inhibitory concentration of essential oils from plant species Origanum vulgare L, Origanum vulgare ssp y Lippia alba Mill cultured in the North of the Department of Bolívar (Colombia), obtained by hydrodistillation and by microwave radiation-assisted hydrodistillation.
Methods: essential oils were extracted by distillation and microwave radiationassisted hydrodistillation from the leaves; relative density at 20°C, refractive index; solubility of the essential oils in ethanol (70% v/v) and optical rotation were all determined. The chemical composition was assessed using gas chromatography/ mass spectrometer The event was perfomed over three bacteria involved in the development of acne: Propionibacterium acnes ATCC 11827, Staphylococcus aureus ATCC 25923 y Staphylococcus epidermidis ATCC 12228. For the purpose of determining the antibacterial sensitivity and the minimum inhibitory concentration, oils were diluted up to reaching the desired concentration (1000-50 µg/mL) using broth microdilution method, and microplate reader for the quantification of bacterial growth.
Results: Yields ranged from 0,04 to 0,16%, depending on the plant species and the extraction method used. The sensitivity test results showed that the bacteria were more sensitive to the essential oil oregano "white border" (Origanum vulgare ssp) obtained by both methods of extraction; in addition, this oil showed the greatest content of oxygenated monoterpenes, with known antibacterial activity, such as carvacrol and thymol.
Conclusions: the essential oil of oregano "white border" (Origanum vulgare ssp) is considered as promising for the control of the bacterial component of acne vulgaris.


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