medigraphic.com
ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2015, Number 4

<< Back

Biotecnol Apl 2015; 32 (4)

Adjusting the conditions of a system for the in vivo production of a nucleopolyhedrovirus of Spodoptera frugiperda (Lepidoptera: Noctuidae)

Ruiz C, Gómez-Valderrama J, Chaparro M, Sotelo P, Villamizar L

Language: English
References: 31
Page: 4311-4316
PDF size: 301.62 Kb.


ABSTRACT

The Colombian Spodoptera frugiperda nucleopolyhedrovirus isolate SfNPV003, was used to develop a biopesticide with a strong potential for the control of this pest in the field, making it an alternative to chemical pesticides. Its marketing has, however, been limited due to the high in vivo virus production costs, which has a significant impact on product price. Hence, in this study, the conditions for the viral multiplication system were adjusted, in order to increase process yield and reduce costs. Initially, the effect of larval age and viral inoculum concentration on viral productivity was determined. Subsequently, the effect of the dietary regime for larvae development was established and finally, the effect of harvest time was evaluated on productivity and contaminant content. Sixteen-day-old larvae (fourth instar) were chosen considering this as an appropriate inoculation age, inoculum concentration was 1 × 107 occlusion bodies (OBs)/mL and a wheat germ-based maintenance diet was selected. Seven days post- inoculation (23 day-old larvae, fourth instar) was chosen as the optimum harvesting time of the infected larvae. Under these conditions the production capacity of viral particles was increased 95 times (from 9.5 × 1011 to 9.1 × 1013 OBs/month), allowing for a 20 % reduction in product price, thus improving its economic feasibility.


REFERENCES

  1. Instituto Colombiano Agropecuario ICA. RESOLUCION No. 000879. Ministerio de Agricultura y Desarrollo Rural. 2008.

  2. Behle RW, Popham HJR. Laboratory and field evaluations of the efficacy of a fast-killing baculovirus isolate from Spodoptera frugiperda. J Invertebr Pathol. 2012;109:194-200.

  3. Caballero P, Murillo R, Muñoz D, Williams T. El nucleopoliedrovirus de Spodoptera exigua (Lepidoptera: Noctuidae) como bioplaguicida: análisis de avances recientes en España. Rev Colomb Entomol. 2009;35:105-15.

  4. Caballero P, Williams T, López-Ferber M. Los baculovirus y sus aplicaciones como bioinsecticidas en el control biológico de plagas. Madrid: España; 2001.

  5. Copping L. The Manual of biocontrol agents, the biopesticide manual. 4th edition; Hampshire: The British Crop Protection Council; 2009.

  6. Grzywacz D, Jones K, Moawad G, Cherry J. The in vivo production of Spodoptera littoralis nuclear polyhedrosis virus. J Virol Methods. 1998;71:115-22.

  7. Sajjan DB, Hinchigeri SB. Increase in production of baculovirus occlusion bodies through cold incubation in silkworms. Int J Adv Res. 2015;3:5-17.

  8. Gómez J, Guevara J, Cuartas P, Espinel C, Villamizar L. Microencapsulated Spodoptera frugiperda nucleopolyhedrovirus: insecticidal activity and effect on arthropod populations in maize. Biocontrol Sci Technol. 2013;23:829-46.

  9. Villamizar L, Barrera G, Cotes AM, Martínez F. Eudragit S100 microparticles containing Spodoptera frugiperda nucleopolyehedrovirus: physicochemical characterization, photostability and in vitro virus release. J Microencapsul. 2010; 27:314-24.

  10. Santos AM, Uribe LA, Ruiz JC, Tabima L, Gómez JA, Villamizar LF. Nucleopoliedrovirus de Spodoptera frugiperda SfNPV003: compatibilidad con agroquí- micos y estabilidad en condiciones de almacenamiento. Corpoica Cienc Tecnol Agropecu. 2014;15:219-28.

  11. Greene G, Leppla N, Dickerson W. Velvetbean caterpillar (Lepidoptera: Noctuidae) rearing procedure and artificial medium. J Econ Entomol. 1976;69:487-8.

  12. Hughes P, Wood H. A synchronous peroral technique for the bioassay of insect viruses. J Invertebr. Pathol. 1981;37:154-9.

  13. Cohen A. Insect diet: science and technology; Boca Raton: CRC Press LLC.; 2004.

  14. Seth RK, Sharma VP. Growth, development, reproductive competence and adult behaviour of Spodoptera litura (Lepidoptera: Noctuidae) reared on different diets. Int Atom Energy Agency Tech Doc IAEA TECDOCs; 2002.

  15. Gómez J, Guevara, Barrera G, Cotes AM, Villamizar L. Aislamiento, identificación y caracterización de nucleopoliedrovirus nativos de Spodoptera frugiperda en Colombia. Rev Fac Nac Agron. 2010;63:1-10.

  16. Dean B, Nishry T. Scoring and profitability models for evaluating and selecting engineering products. J Oper Res Soc Am. 1965;13:550-69.

  17. Gilbert L. Insect development morphogenesis, molting and metamorphosis. Oxford: Academic Press; 2009.

  18. Busato GR, Garcia MS, Loeck AE, Zart M, Nunes AM, Bernardi O, et al. Adequacao de uma dieta artificial para os biótipos “milho” e “arroz” de Spodoptera frugiperda (Lepidoptera: Noctuidae). Bragantia. 2006; 65:317-23.

  19. Lee KP, Simpson SJ, Wilson K. Dietary protein-quality influences melanization and immune function in an insect. Funct Ecol. 2008;22:1052-61.

  20. Wiseman B, Carpenter E. Growth inhibition of corn earworm (Lepidoptera: Noctuidae) larvae reared on resistant corn silk diets. J Econ Entomol. 1995;88:1037-43.

  21. Lee KP, Simpson SJ, Raubenheimer D. A comparison of nutrient regulation between solitarious and gregarious phases of the specialist caterpillar, Spodoptera exempta (Walker). J Insect Physiol. 2004;50: 1171-80.

  22. Pomeranz Y, Meloan C. Food analysis: Theory and practice; 3rd edition. New York: Chapman and Hall; 1994.

  23. Karowe DN, Martin MM. The effects of quantity and quality of diet nitrogen on the growth, efficiency of food utilization, nitrogen budget, and metabolic rate of fifth-instar Spodoptera eridania larvae (Lepidoptera: Noctuidae). J Insect Physiol. 1989;35:699-708.

  24. Popham HJR, Shelby KS. Uptake of dietary micronutrients from artificial diets by larval Heliothis virescens. J Insect Physiol. 2006;52:771-7.

  25. Murúa MG, Virla EG, Defagó V. Evaluacion de cuatro dietas artificiales para la cría de Spodoptera frugiperda (Lep.: Noctuidae) destinada a mantener poblaciones experimentales de himenópteros parasitoides. Bol San Veg Plagas. 2003;29:43-51.

  26. Kalia V, Chaudhari S, Gujar GT. Optimization of production of nucleopolyhedrovirus of Helicoverpa armigera throughout larval stages. Phytoparasitica. 2001;29:23-8.

  27. Grzywacz D, Rabindra RJ, Brown M, Jones KA, Parnell M. The Helicoverpa armigera NPV production manual. 2011 [cited 2015 Jun 15]. Available from: www.fao. org/docs/eims/upload/agrotech/2011/ hanpvmanual-pt1.pdf

  28. Adamo S. The impact of physiological state on immune function in insects. In: Rolff, J.; Reynolds, S., Eds. Insect Infection and Immunity. Evolution, Ecology and Mechanisms. Oxford: Oxford University Press; 2009; p. 173-86.

  29. Cherry J, Parnell M, Grzywacz D, Jones K. The optimization of in vivo nuclear polyhedrosis virus production in Spodoptera exempta (Walker) and Spodoptera exigua (Hubner). J Invertebr Pathol. 1997; 70:50-8.

  30. Caballero P, Williams T. Virus entomopatógenos. In: Control biológico de plagas agrícolas; Valencia: Editorial Phytoma; 2008. p. 121-35.

  31. Ravensberg W. Progress in biological control. A roadmap to the successful development and commercialization of microbial pest control products for control of arthropods. Netherlands: Springer; 2011.




2020     |     www.medigraphic.com