Cerezo LG, Zárate CK, Alpuche-Lazcano S, Cabello C, Tato P, Tirado R

Language: Spanish
References: 33
Page: 88-95
PDF size: 475.17 Kb.

ABSTRACT

In Mexico the molecular epidemiology and viral etiology of acute respiratory infections are very limited; there are no data about the frequency and circulation patterns of different seasonal human Metapneumovirus. Therefore, we design a molecular technique to detect and identify human Metapneumovirus. A total of 118 clinical samples from pediatric patients with low respiratory tract infections were isolated and classified by their fusogenic activity as fusogenic and non fusogenic isolates. Moreover, fusogenic and non fusogenic isolates were human Metapneumovirus confirmed by direct immunofluorescence. The human metapneumovirus positive isolates (42/118) were confirmed by RT-PCR using oligonucleotides designed in our laboratory to amplify the viral genes N (Nucleocapsid) and/or L (RNA polimerase). We demonstrated by RT-PCR that human Metapneumovirus infection was detected in 100% of the viral isolates previously confirmed as positive by direct immunofluorescence assay (42/118). Alternatively, from the negative isolates by direct immunofluorescence (17), we were able to identify seven isolates as human respiratory syncytial virus using our designed RT-PCR.

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