Cerezo LG, Zárate CK, Alpuche-Lazcano S, Cabello C, Tato P, Tirado R

Idioma: Español
Referencias bibliográficas: 33
Paginas: 88-95
Archivo PDF: 475.17 Kb.

RESUMEN

Actualmente no se conoce en México la epidemiología de metapneumovirus humano, por lo que se implementó una metodología molecular para detectar y diagnosticar metapneumovirus humano. A partir de un total de 118 pacientes en edad pediátrica con infección respiratoria aguda, se tomaron muestras clínicas, de las cuales se obtuvieron aislados virales que se clasificaron en fusogénicos y no fusogénicos en función de su capacidad de inducir o no la formación de sincitios. Posteriormente, los aislados virales se sometieron a detección de metapneumovirus humano por inmunofluorescencia directa y se analizaron por RT-PCR con oligonucleótidos específicos para la amplificación de los genes N (nucleocápside) y/o L (RNA polimerasa viral). Los resultados obtenidos demostraron que mediante RT-PCR con nuestro diseño de oligonucleótidos identificamos como metapneumovirus humano positivos el 100% de los aislados virales metapneumovirus humano positivos por inmunofluorescencia directa (42/118). Cabe destacar que con esta metodología se detectaron siete muestras hRSV positivas mediante RT-PCR a partir del grupo de 17 muestras metapneumovirus humano negativas por inmunofluorescencia directa, lo cual pone de manifiesto la mayor sensibilidad de nuestra metodología.

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