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ISSN 2007-4360 (Print)
Asociación Mexicana de Periodontología, Colegio de Periodoncistas A.C

2015, Number 3

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Rev Mex Periodontol 2015; 6 (3)

Characterization of biofilm in the apical third of dental organs removed because of periodontal disease

Nava HSM, Narváez DJD, González AAM, Aragón PA, Araujo AC, Zermeño IJA

Language: Spanish
References: 44
Page: 110-120
PDF size: 555.40 Kb.


ABSTRACT

Background: Microorganisms present in the oral cavity are not usually found in planktonic form, but in communities called biofilm, which offer great resistance against antimicrobial agents. Biofilm can be present in several areas of a tooth where an infection is found, and if such infection reaches the pulp or the periodontal tissues, it is possible that an exchange of toxic agents could take place. The bacterial interactions present in the periodontal disease and their relation with the root canal system are of great concern, as well as to match them with the presence of biofilm. Objectives: The aim of this study was to identify and categorize the microorganisms present in the biofilm formed on the apical third of teeth extracted due to periodontal disease by using conventional microbiological techniques, scanning electron microscopy (SEM) and Raman spectroscopy. Material and methods: A total of 20 teeth previously diagnosed with periodontal disease and mobility class III were extracted. The informed consent was previously signed at the Periodontal Department of the Dental School at Universidad Autónoma de San Luis Potosí (UASLP). Samples of the apical third were sliced longitudinally and divided into two groups: 1) extraradicular analysis; 2) root canal system analysis. Raman spectroscopy was also performed. Conclusions: Presence of biofilm was observed both within and outside the root canal samples. A match was observed in the microorganisms present in group 1 compared to group 2. Main chemical composition was determined by vibrational spectroscopy.


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