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2015, Number 3

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VacciMonitor 2015; 24 (3)

Molecular characterization of a Cuban isolation of canine parvovirus

Fresneda-Disotuar A, Contreras-González D, Paneque-Zayas M, de la Cruz-Verdura L, Veda RC

Language: Spanish
References: 20
Page: 113-119
PDF size: 243.23 Kb.


ABSTRACT

The type 2 canine parvovirus (CPV-2) is the causal agent of an infected-contagious disease that produces a hemorrhagic and acute gastroenteritis that affects young canines. The CPV-2 is a virus with a small, naked DNA genome, very resistant to the environmental conditions that emerged and expanded fast at the end of the 70´s decade. In the 1980, two antigenic variants of CPV-2 emerged almost simultaneously. They were called CPV-2a and CPV-2b. In the 2000, a new antigenic variant was detected. It was called CPV-2c, and was frequently reported in canine communities in some countries worldwide. The objective of this work is to characterize a Cuban isolation of canine parvovirus attenuated and adapted to cellular cultivation. The genetic material was amplified by polymerase chain reaction and sequencing later. The obtained sequences were analyzed and compared with sequences of well-known strains placed in the data base where it was evidenced that the Cuban isolation was of CPV-2 type.


REFERENCES

  1. Hoelzer K, Parrish, C. The emergence of parvoviruses of carnivores. Vet Res 2010;41(6):39. doi: 10.1051/vetres/2010011.

  2. Castro TX, Costa EM, Leite JP, Labarthe NV, Garcia RC. Detection of the new variant CPV-2c. Braz J Microbiol 2010;41(4):1093-8.

  3. Cavalli A, Martella V, Desario C, Camero M, Bellacicco A, De Palo P, et al Evaluation of the antigenic relationships among canine parvovirus type 2 variants. Clin Vacc Immunol 2008;15(3):534-9.

  4. Hoelzer K, Shackelton LA, Parrish CR, Holmes EC. Phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses. J Gen Virol 2008;89(9):2280-9.

  5. Maya L, Calleros L, Francia L, Hernández M, Iraola G, Panzera Y, et al. Phylodynamics analysis of canine parvovirus in Uruguay: evidence of two successive invasions by different variants. Arch Virol 2013;158(6):1133-41.

  6. Calderón MG, Romanutti C, D'Antuono A, Keller L, Mattion N, La Torre J. Evolution of Canine Parvovirus in Argentina between years 2003 and 2010: CPV2c has become the predominant variant affecting the domestic dog population. Virus Res 2011;157(1):106-10.

  7. De Cramer KG, Stylianides E, Van Vuuren M. Efficacy of vaccination at 4 and 6 weeks in the control of canine parvovirus. Vet Microbiol 2011;149(1-2):126-32.

  8. Merino N, Rodríguez J, Joa R, Achon I, Cabo JL. Reporte de un brote de gastroenteritis hemorrágica canina. Rev Cub Cienc Ve. 1982;13(2):223-4.

  9. López R. Parvovirosis canina. La Habana: Universidad Agraria de la Habana; 1998.

  10. Boffil P, Ramírez W, Montañez J, Reinaldo G, Perez M, Percedo M, Aveledo MA. Enfermedades Infecciosas de los animales. Enfermedades producidas por virus. Tomo II. La Habana: Editorial Félix Varela; 2007. p.341-62.

  11. Puentes R. Parvovirosis Canina: situación actual y protección de vacunas contra las nuevas variantes virales circulantes en la región. Veterinaria 2012;48(185):5-10.

  12. Barrios M, Luya M, Reyna A, Lorenzo M, Achon I. Aislamiento de parvovirus de un perro con gastroenteritis hemorrágica. Rev Cub Cienc Vet 1989;20(4):297-304.

  13. Reed PA, Jones EV, Miller TJ. Nucleotide Sequence and Genome Organization of Canine Parvovirus. J Virol. 1988;62:266-76.

  14. Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics. 2004;5:150-63.

  15. Zhang Z, Schwartz S, Wagner L, Webb Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000;7(1-2):203-14.

  16. Truyen U. Evolution of canine parvovirus – A need for new vaccines? Microbiol 2006;117:9-13.

  17. Pratelli A, Cavalli A, Normanno G, De Palma MG, Pastorelli G, Martella V, Buonavoglia C. Immunization of pups with maternally derived antibodies to canine parvovirus (CPV) using a modified live variant (CPV-2b). J Vet Med B Infect Dis Vet Public Health. 2000;47:273-6.

  18. Greenwood NM, Chalmers WSK, Baxendale W, Thompson H. Comparison of isolates of canine parvovirus by restriction enzyme analysis and vaccine efficacy against field strains. Vet Rec 1995;136:63-7.

  19. Spibey N, Greenwood NM, Sutton D, Chalmers WS, Tarpey I. Canine parvovirus type 2 vaccine protects against virulent challenge with type 2c virus. Vet. Microbiol. 2008;128(1-2):48-55.

  20. Truyen U, Everman J, Vieler E, Parrish C. Evolution of canine parvovirus involved loss and gain of feline host range. Virology 1996;215:186-9.




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