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2002, Number 4

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Rev Inst Nal Enf Resp Mex 2002; 15 (4)

Thymus-and activation-regulated chemokine (TARC) is released in the airways of asthmatic patients.

Lezcano MD, Negrete GMC, Montes VAR, Valencia MEL, García-Sancho FCE, Franco MF, Terán JLM

Language: Spanish
References: 19
Page: 201-208
PDF size: 59.93 Kb.


ABSTRACT

Introduction: The presence of Th2 lymphocytes in airways of bronchial asthma patients is a well-recognized feature. Thymus- and activation- regulated chemokine (TARC) is a potent chemoattractant for Th2 lymphocytes and plays an important role in inflammatory diseases. Objective: TARC release in the bronchoalveolar lavage (BAL) fluid from asthmatic patients was investigated. Material and methods: BAL was performed to 17 asthmatics and 13 controls. Results: Differential count and TARC measurement by ELISA was performed. Differential cell counts in BAL fluid showed that numbers of lymphocytes, eosinophils and epithelial cells were significantly elevated in asthmatic subjects compared with controls (6.1 vs 1.0 lymphocytes x 103/mL, 1.4 vs 0.24 eosinophils x 103/mL and 1.3 vs 0.02 epithelial cells x 103/mL, respectively). Measurements of TARC by ELISA showed that levels of this cytokine were significantly elevated in BAL fluid from asthmatics compared with normal subjects (median 313 vs 35 pg/mL). There was a significant correlation between TARC levels in BAL fluid and bronchial hyperreactivity (PC20 response to metacholine) (r= -0.7, p ‹0.001) and between TARC levels and lymphocytes numbers in BAL fluid (r= 0.47, p= 0.001). Discussion: These findings suggest that TARC may play an important role in the pathogenesis of asthma.


REFERENCES

  1. Ying S, Humbert M, Barkans J, Corrigan CJ, Pfister R, Menz G, et al. Expression of IL-4 and IL-5 mRNA and protein product by CD4+ and CD8+ T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsec) asthmatics. J Immunol 1997; 158:3539-3544.

  2. Lai CK, Ho SS, Chan CH, Leung R, Lai KN. Gene expression of interleukin-3 and granulocyte macrophage colony-stimulating factor in circulating CD4+ T cells in acute severe asthma. Clin Exp Allergy 1996; 26:138-146.

  3. Mosmann TR, Sad S. The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today 1996;17:138-146.

  4. Luster AD. Chemokines: chemotactic cytokines that mediate inflammation. N Engl J Med 1998;338:436-445.

  5. Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity. Immunity 2000;12:121-127.

  6. Imai T, Yoshida T, Baba M, Nishimura M, Kakizaki M, Yoshie O. Molecular cloning of a novel T cell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. J Biol Chem 1996;271:21514-21521.

  7. Kawasaki S, Takizawa H, Yoneyama H, Nakayama T, Fujisawa R, Izumizaki M, et al. Intervention of thymus and activation-regulated chemokine attenuates the development of allergic airway inflammation and hyperresponsiveness in mice. J Immunol 2001;166:2055-2062.

  8. Berin MC, Eckmann L, Broide DH, Kagnoff MF. Regulated production of the helper 2-type T-Cell chemoattractant TARC by human bronchial epithelial cells in vitro and in human lung xenografts. Am J Respir Cell Mol Biol 2001; 24:382-389.

  9. Sekiya T, Miyamasu M, Imanishi M, Yamada H, Nakajima T, Yamaguchi M, et al. Inducible expression of a Th2-type CC chemokine thymus- and activation-regulated chemokine by human bronchial epithelial cells. J Immunol 2000;165: 2205-2213.

  10. National Heart, Lung and Blood Institute. Workshop summary and guidelines: investigative use of bronchoscopy, lavage and bronchial biopsies in asthma. J Allergy Clin Immunol 1991; 88:808-814.

  11. Romagnani S. Cytokines and chemoattractants in allergic inflammation. Mol Immunol 2001;38:8881-8885.

  12. Holgate S, Lackie M, Davies D, Roche W, Walls F. The bronchial epitheliumas a key regulator of airway inflammation and remodelling in asthma. Clin Exp Allergy 1999;29 (2 Suppl):90S.

  13. Sekiya T, Yamada H, Yamaguchi M, Yamamoto K, Ishii A, Yoshie O, et al. Increased levels of a TH2-type CC chemokine (TARC) in serum and induced sputum of asthmatics. Allergy 2002;57:173-177.

  14. Sugawara N, Yamashita T, Ote Y, Miura M, Terada N, Kurosawa M. TARC allergic disease. Allergy 2002;57:180-181.

  15. Imai T, Nagira M, Takagi S, Kakizaki M, Nishimura M, Wang J, et al. Selective recruitment of CCR4-bearing Th2 cells toward antigen –presenting cells by the CC chemokines thymus and activation –regulated chemokine and macrophage-derived chemokine. Int Immunol 1999;11:81-88.

  16. Panina-Bordignon P, Papi A, Mariani M, Di Lucia P, Casoni G, Bellettato C, et al. The C-C chemokine receptors CCR4 and CCR8 identify airway T cells of allergen-challenged atopic asthmatics. J Clin Invest 2001;107:1357-1364.

  17. Ryan JJ. Interleukin-4 and its receptor: Essential mediators of the allergic response. J Allergy Clin Immunol 1997;99:1-5.

  18. Brombacher F. The role of interleukin-13 in infectious diseases and allergy. Bioassays 2000;22:646.

  19. Ying S, Meng Q, Barata LT, Robinson DS, Durham SR, Kay AB. Associations between IL-13 and IL-4 (mRNA and protein), vascular cell adhesion molecule-1 expression, and the infiltration of eosinophils, macrophages, and T cells in allergen-induced late-phase cutaneous reactions in atopic subjects. J Immunol 1997;158:5050-5057.




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