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ISSN 1561-3054 (Electronic)

2015, Number 1

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Rev Cubana Med Trop 2015; 67 (1)

Usefulness of the nitrate reductase method to detect resistance to second-line anti-tuberculosis drugs

Lemus MD, Álvarez EY, Echemendía FM, Van der Stuyft P, Palomino JC, Martin A

Language: Spanish
References: 17
Page: 20-27
PDF size: 109.61 Kb.


ABSTRACT

Introduction: The increase of multidrug resistant and extensively drug resistant tuberculosis underlines the urgent need to obtain early results of Mycobacterium tuberculosis susceptibility both to first and second line antituberculosis drugs in order to avoid dissemination of resistant isolates.
Objective: The aim of this research was to compare the performance of the nitrate reductase assay and the proportion method for to detect the susceptibility to second line antituberculosis drugs in multidrug resistant clinical isolates of M. tuberculosis.
Methods: The susceptibility to ofloxacin, kamamycin and capreomycin of 34 M. tuberculosis multidrug resistant isolates was investigated using the proportion method in Löwenstein-Jensen and the nitrate reductase assay.
Results: Three isolates were identified as extensively drug resistant by both methods. The results of the nitrate reductase assay were obtained between 7-14 days achieving 100 %, 90.0 % and 77.8 % of sensitivity for ofloxacin, kamamycin and capreomycin, respectively while specificity was higher than 95.0 % and kappa value was higher to 0,85 for all drugs.
Conclusion: The nitrate reductase assay represents a useful tool for the rapid identification of extensively drug resistant tuberculosis in low resources setting.


REFERENCES

  1. World Health Organization. Global tuberculosis control: WHO report 2013. Geneva: WHO; 2013.

  2. Montoro E, Lemus D, Echemendia M, Díaz R, Mederos L, Martinez MR, et al. Vigilancia de la resistencia a los fármacos antituberculosos en Cuba, 2000-2009. Rev Panamericana Salud Pública 2011;30:615-8.

  3. Lemus D, Echemendía M, Díaz R, Llop A, Llanes MJ. Vigilancia de la resistencia a los medicamentos antituberculosos en Cuba, 2010-2011. Biomédica 2014;34 (Suppl 1):108-13.

  4. Palomino JC. Current developments and future perspectives for TB diagnostics. Future Microbiol 2012;7:59-71.

  5. Canetti G, Fox W, Khomenko A, Mahler HT, Menon NK, Mitchison DA, et al. Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ 1969;41:21-43.

  6. Angeby KA, Klintz L, Hoffner SE. Rapid and inexpensive drug susceptibility testing of Mycobacterium tuberculosis with a nitrate reductase assay. J Clin Microbiol 2002;40:553-5.

  7. Montoro E, Lemus D, Echemendía M, Martin A, Portaels F, Palomino JC. Comparative evaluation of the nitrate reduction assay, the MTT test and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis. J Antimicrob Chemother 2005;55:500-5.

  8. Martín A, Montoro E, Lemus D, Simboli N, Morcillo N, Velasco M, et al. Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis. J Microbiol Methods 2005;63:145-50.

  9. Lemus D, Montoro E, Echemendia M, Martin A, Portaels F, Palomino JC. Nitrate Reductase Assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low resources laboratories. J Med Microbiol 2006;55:861-3.

  10. World Health Organization. Noncommercial culture and drug-susceptibility testing methods for screening patients at risk for multidrug resistant tuberculosis. WHO/HTM/TB/2011.9. Geneva: WHO; 2011.

  11. Mirabal N, Yzquierdo S, Lemus D, Madruga M, Milian Y, Echemendía M, et al. Evaluation of Colorimetric Methods Using Nicotinamide for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis. J Clin Microbiol 2010;48:2729-33.

  12. World Health Organization. Global tuberculosis control: WHO report 2012. Geneva: WHO; 2012.

  13. Martin A, Palomino JC, Portaels F. Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays. J Clin Microbiol 2005;43:1612-6.

  14. Rosales S, Pineda L, Andino N, Almendarez N, Membreño H, Hoffner SE. Evaluation of the nitrate reductase assay for rapid detection of extensively drugresistant tuberculosis. Int J Tuberc Lung Dis 2009;13:1542-9.

  15. Devasia RA, Blackman A, May C, Eden S, Smith T, Hooper N, et al. Fluoroquinolone resistance in Mycobacterium tuberculosis: an assessment of MGIT 960, MODS and nitrate reductase assay and fluoroquinolone cross-resistance. J Antimicrob Chemother 2009;63:1173-8.

  16. Imperiale BR, Morcillo NS, Palomino JC, Vandamme P, Martin A. Predictive value of direct nitrate reductase assay and its clinical performance in the detection of multi and extensively drug resistant tuberculosis. J Med Microbiol 2014;63:522-7.

  17. Martin A, Imperiale B, Ravolonandriana P, Coban AY, Akgunes A, Ikram A, et al. Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis. J Antimicrob Chemother 2014;69:441-4.




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