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2001, Number 1

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Rev Inst Nal Enf Resp Mex 2001; 14 (1)

Diagnosis of tuberculosis by detection of Mycobacterium tuberculosis with a non-commercial polymerase chain reaction.

Laniado-Laborín R, Enríquez-Rosales ML, Licea NAF

Language: Spanish
References: 13
Page: 22-25
PDF size: 154.97 Kb.


ABSTRACT

Objective: To evaluate the efficacy of a non-commercial direct amplification test based on the polymerase chain reaction to identify Mycobacterium tuberculosis in patients with presumptive diagnosis of pulmonary tuberculosis and smear-positive sputum. Material and methods: Sputum and pleural fluid samples were analysed with the direct amplification technique, using the sequences 5’CAGCCCGCTGGAGTCCCGCGGA3’ and 5’GCGCGTTCACCATGGACACCG3’ as primers and a Lowenstein-Jensen culture as gold standard. Results: Seventy-nine consecutive patients and a total of 212 specimens were analysed. Using the gold standard, PCR sensitivity in sputum samples was 97% and positive predictive value, 57%. PCR was positive in 54 (98.18%) of the 55 specimens with positive septum microscopy. Conclusions: Sensitivity and positive predictive value of the non-commercial primers used to identify Mycobacterium tuberculosis in smear-positive sputum are comparable to reported clinical studies. Its clinical application will allow rapid identification of Mycobacterium tuberculosis infected patients, discerning them from patients infected with non-tuberculous mycobacteria.


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