2015, Number 4
<< Back Next >>
Rev Cub Med Int Emerg 2015; 14 (4)
Klebsiella pneumoniae and Escherichia coli as betalactamasas producers in patients with urinary tract infection
Argüez PAR, Rodríguez CA, Rojas HN
Language: Spanish
References: 32
Page: 16-29
PDF size: 457.64 Kb.
ABSTRACT
Introduction: The increment of antimicrobial resistance in enterobacterium stumps is a worldwide problem, particularly
Escherichia coli and
Klebsiella pneumoniae, due to the antibiotic therapies abuse; becoming a great challenge for medicine and technological improvements.
Objective: To determine the extended spectrum betalactamasas (ESBL) frequency in
Escherichia coli and
Klebsiella pneumoniae isolations in patient with urinary tract infection (UTI).
Method: Descriptive and observational study that it included from February 2011 through July 2013 period. The sample was 150 positive urocultivos about patients assisted in the Hospital's Urology service; 73 of them were confirmed the
Escherichia coli and
Klebsiella pneumoniae BLEE producers presence.
Results: The studied showed that
Escherichia coli and
Klebsiella pneumoniae stumps constituted the higher isolation percentage found in patients with UTI translated to the Urology service. ESBL and acetiltransferas (6') AAC´ enzymes, were the bigger responsible for the
Escherichia coli and the
Klebsiella pneumoniae stumps antimicrobial resistance in the patients studied. The previous use of antibiotics and the renal lithiasis were the most common risk factors present in the ESBL producer’s bacterium isolations.
Conclusions: Antimicrobial resistance is a nowadays problem that requires special attention by clinical physicians, microbiologists and other professionals in order to diagnose and treat the infections; that is why it is necessary to know that
Escherichia coli and the
Klebsiella pneumoniae are the most frequent ESBL producer’s stumps in the patients remitted from the Urology service.
REFERENCES
Livermore D. M., It’s the era of untreatable infections arrived? J. Antimicrob. Chemotherapy. 64, i29–i36 (2009).
Roca GR. y col., Temas de Medicina Interna La Habana: Editorial Ciencias Médicas; 4 ed.121-125(2007).
Wright G. D., The antibiotic resists me: the nexus of chemical and genetic diversity. Nature Rev. Microbiol. 5,175–186 (2007) y Falagas ME, Karageorgopoulos DE. Extended-spectrum beta-lactamase-producing organisms. J Hosp Infect. 2009;73:345-54.
Kaier K, Frank U, Conrad A, Meyer E. Seasonal and ascending trends in the incidence of carriage of extended-spectrum betalactamase-producing Escherichia coli and Klebsiella species in 2 German hospitals. Infect Control Hosp Epidemiol. 2010;31:1154-9.
Procedimientos Técnicos de Microbiología Clínica. 2004.
Vandepitte J., 1993: Métodos Básicos de Laboratorio en Bacteriología Clínica. Organización Mundial de la Salud. Ginebra. Suiza.
Famiglietti A, Quinteros M, Vauez M. Consenso sobre las pruebas de Microbiol.2005; 37(1):57-66.
BioMérieux.com [página web en Internet]. Argentina; c 1996-2012 [actualizado 4 de abril del 2012; citado 5 de mayo del 2012]. Disponible en: http://www.biomerieux.com.ar/
Remedios H. M., Cheping S. N., Temas de Urología. Editorial Ciencias Médicas, 2008.
Silva J. Resistencia a antibióticos .Rev. Latinoamericana de Microb. 2006; 48:105-112.
Willems E, Verhaegen J, Magerman K, Nys S, Cartuyvels R. Towards a phenotypic screening strategy for emerging β-lactamases in Gram-negative bacilli. Int J Antimicrobial Agents. 2013; 41:99-109.
Li XM, Jang SJ, Bae IK, Park G, Kim YS, Shin JH, et-al. [Frequency of extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase genes in Escherichia coli and Klebsiella pneumoniae over a three-year period in a University Hospital in Korea]. Korean J Lab Med. 2010;30:616-23.
Schoevaerdts D, Bogaerts P, Grimmelprez A, Saint Hubert M, Delaere B, Jamart J. Clinical profiles of patients colonized or infected with extended spectrum betalactamase producing Enterobacteriaceae isolates: a 20 month retrospective study at a Belgian University Hospital. BMC Infect Dis. 2011;11:12.
Suárez Trueba Bettsy, Hart Casares Marcia, Ramírez Margarita, Salazar Rodríguez Daniel .Detección de fenotipos de resistencia en aislamientos de Klebsiella pneumoniae y Escherichia coli multidrogorresistentes. Hospital Clínico quirúrgico "Hermanos Ameijeiras". 2010.
Espinosa F, Hart C M, Halley M, Martínez M, Pardo AV. Resistencia bacteriana de cepas aisladas en el Hospital "Hermanos Ameijeiras". Rev. Cubana med v.47n.4 Ciudad de la Habana oct-dic. 2010; 10: 597–602.
Villegas M, Correa A, Pérez F, Miranda M, Zuloaga T, Quinn J. Colombiano Nosocomial Resistance Study Group. Prevalence and characterization of extended-spectrum betalactamases in Klebsiella pneumoniae and Escherichia coli isolates from Colombian hospitals. Diagn Microbiol Infect Dis. 2004; 49:217-22.
Vergidis PI, Falagas ME. Multidrug-resistant gramnegative bacterial infections: The emerging threat and potential novel treatment options. Curropin Invest Drugs. 2008; 9:176-83.
Rodríguez Villalobos H, Bogaerts P, Berhin C, Bauraing C, Deplano A, Montesinos I. Trends in production of extended spectrum beta lactamases among Enterobacteriaceae of clinical interest: results of a nationwide survey in Belgian hospitals. J Antimicrob Chemother. 2011;66:37-47.
Pfaller Ma, Segret J Overview of the epidemiological profile and laboratory detection of extended spectrum betalactamases.CID. 2006; 42:153-63.
Treviño M, Martínez L, Romero –Jung P, Varón C, Molde L, García-Riestra C. Comparación entre las pruebas para la detección de betalactamasas de espectro extendido de los sistemas Vitek 2 y Phoenix. Enfer Infecc Microbiol Clin. 2009; 27:566-70.
Taneja J, Mishra B, Thakur A, Dogra V, Loomba P. Nosocomial blood-stream infections from extended spectrum betalactamase producing Escherichia coli and Klebsiella pneumoniae from GB Pant Hospital, New Delhi. J Infect Dev Ctries. 2010;4:517-20.
Coque TM., Baquero F, Canton R. Increasing prevalence of ESBL producing Enterobacteriaceae in Europe. Euro Surveill. 2008.
Hart Casares Marcia, Espinosa Rivera Fidel, Halley Posada María del Carmen, Martínez Batista María Luisa, Montes de Oca Méndez Zurelys. Resistencia a antibióticos en cepas de Acinetobacter baumannii aisladas de enero a marzo del 2010 en el Hospital Clínico quirúrgico "Hermanos Ameijeiras". Rev. cubana med 2012; 49(3):218-227.
Pedro W, Ramos A, Nodarse R, Padrón A, De armas E. Resistencia bacteriana en las bacterias productoras de betalactamasas extendidas (BLEE). Revista Cubana de Medicina Intensiva y Emergencias 2006; 5(1):294-301.
Suárez CJ, Kattan JN, Guzmán AM, Villegas MV. Mecanismos de resistencia a carbapenems en P. aeruginosa, Acinetobacter y Enterobacteriaceae y estrategias para su prevención y control. Infect. 2006; 10(2):85-93.
Calvo J, Cantón R, Fernández-Cuenca F, Mirelis B, Navarro F. Detección fenotípica de mecanismos de resistencia en gramnegativos. En: Cantón R, Cercenado E, editores. Procedimientos en Microbiología Clínica Recomendaciones de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. 2. a ed. (38) 2011. Disponible en: http://www.seimc.org/documentos/protocolos/microbiología/
Pitout JD. Infections with extended spectrum betalactamase producing enterobacteriaceae: changing epidemiology and drug treatment choices. Drugs. 2010;70:313-33.
Navarro F, Calvo J, Cantón R, Fernández-Cuenca F, Mirelis B. Detección fenotípica de mecanismos de resistencia en microorganismos gramnegativos. Enferm Infecc Microbiol Clin. 2011; 29:524-34.
Pitout JD, Gregson D, Campbell L, Laupland K Molecular Characteristics of extended spectrum betalactamase producing Escherichia coli isolates causing bacteremia in the Calgary health region from 2000 to 2007 .Antimicrobial Agents and Chemotherapy.2009; 53(7):2846-5.
Jacoby GA. Amp C betalactamases. Clin Microbiol Rev 2009; 22:161-82.
González Vélez, Abel Ernesto; Díaz Agero Pérez, Cristina; Robustillo-Rodela, Ana; Pita López, María José; Cornejo Gutiérrez, Ana María; Pedrero Pérez,Pelayo;Monge Jodra,Vicente; Publicado en Med Clin (Barc).Tendencia de la prevalencia de bacilos gramnegativos productores de betalactamasas de espectro extendido en un hospital universitario de Madrid. 2013; 141:8-12.
Velasco AM, Barrena PR, Asenjo MA, Valverde Cánovas JF, Delgado-Iribarren A, Losa García JE. Risk factors for extended espectrum beta-lactamase producer E.coli bacteremia from urinary origin. Med Clin (Barc). 2010;134:392.