Salgado-Albarrán M, Herrera-Díaz J, Dinkova TD

Language: Spanish
References: 20
Page: 43-51
PDF size: 741.69 Kb.

ABSTRACT

A group of storage proteins highly abundant in cereal seeds are the prolamins, characterized by the frequent presence of proline in their sequence. The barley prolamins are known as hordeins. The aim of this study was to obtain the hordein banding patterns for five different Mexican barley cultivars in mature seeds. In addition, the hordein patterns in processed malt of four mexican and a canadian (Metcalfe) barley cultivars were obtained. Mass Spectrometry (MS), using distinct digestion protocols, identified differential bands in the patterns. Major differences in the seed patterns between cultivars consisted in discrete bands at 100 kDa, 65 kDa and in the range of 37 to 45 kDa. In malt, the patterns were highly contrasting among Mexican cultivars as well as in Metcalfe, suggesting that hordein processing during germination and malt processing is particular to each cultivar. Finally, the MS identification demonstrated that trypsin digestion is appropriate to distinguish B and γ hordeins in malt, whereas sequential digestion with chymotrypsin and trypsin allows the identification of C hordeins in seed.

REFERENCES

1. Baik, B. K. & Ullrich, S. Barley for food: Characteristics, improvement, and renewed interest. J. Cereal Sci. 48, 233- 242 (2008).

2. Shewry, P. R., Napier, J. A. & Tatham, A. S. Seed Storage Proteins: Structures and biosynthesis. Plant Cell. 7, 945-956 (1995).

3. Colgrave, M. L., Goswami, H., Howitt, C. A. & Tanner, G. J. What is in a Beer? Proteomic Characterization and Relative Quantification of Hordein (Gluten) in Beer. J. Proteome Res. 11, 386-396 (2012).

4. Briggs, D. E., Boulton, C. A., Brookes, P. A. & Stevens, R. Brewing, Science and Practice (CRC Press, Boca Raton Fl., USA, 2004).

5. Legge, W. G. et al. AC Metcalfe barley, cultivar description. Can. J. Plant Sci. 83, 381-384 (2002).

6. Fernández-Vera, Z. Nuevas variedades de cebada cervecera adaptadas a México. (Impulsora Agrícola, México, 2013).

7. Shewry, P. R. & Tatham, A. S. The prolamin storage proteins of cereal seeds: structure and evolution. Biochem. J. 267, 1-12 (1990).

8. Rechinger, K. B., Simpson, B.J., Svendsen, I. & Cameron-Mills, V. A role for γ3 hordein in the transport and targeting of prolamin polypeptides to the vacuole of developing barley endosperm. The Plant J. 4, 841-853 (1993).

9. Wallace, J. C., Lopes, M. A., Paiva, E. & Larkins, B.A. New methods for extraction and quantification of zeins reveal a high content of gamma-zeins in modified opaque-2 maize. Plant Physiol. 92, 191-196 (1990).

10. Yin, T. Q., Ma, D. Q. & Ding, Y. Analysis of genetic diversity of hordein in wild close relatives of barley from Tibet. Theor. Appl. Genet. 107, 837-842 (2003).

11. Agrawal, G. & Thelen, J. A high resolution two dimensional gel-and Pro-Q DPS-Based proteomics workflow for phosphoprotein identification and quantitative profiling. Methods Mol. Biol. 3, 834-841 (2009).

12. Echart-Almeida, C. & Cavalli-Molina, S. Hordein polypeptide patterns in relation to malting quality in Brazilian barley varieties. Pesq. Agropec. Bras. 36, 211-217 (2001).

13. March, T. J., et al. Identification of proteins associated with malting quality in a subset of wild barley introgression lines. Proteomics. 12, 2843-2851 (2012).

14. Rahman, S., Shewry, P. R., Forde, B. G., Kreis, M. & Miflin, B. J. Nutritional control of storage-protein synthesis in developing grain of barley (Hordeum vulgare L.). Planta. 159, 366-371 (1983).

15. Qi, J. C., Zhang, G. P. & Zhou, M. X. Protein and hordein content in barley seeds as affected by nitrogen level and their relationship to beta-amylase activity. J. Cereal Sci. 42, 102-107 (2006).

16. Qi, J. C. et al. Protein and hordein fraction content in barley seeds as affected by sowing date and their relations to malting quality. J. Zhejiang Univ. Sci. 11, 1069-1075 (2005).

17. Flodrová, D., Ralplachta, J., Benkovská, D. & Bobálová, J. Application of proteomics to hordein screening in the malting process. Eur. J. Mass. Spectrom. 18, 323-332 (2012).

18. Jin, B. et al. Investigation of the relationship of malt protein and beer haze by proteome analysis. J. Food Process. Press. 36, 169-175 (2012).

19. Berne, L. J. Endoproteases of barley and malt. J. Cereal Sci. 42, 139-156 (2005).

20. Osman, A. M. et al. Characterization and Assessment of the Role of Barley Malt Endoproteases During Malting and Mashing. J. Inst. Brew. 108, 62-67 (2002).