medigraphic.com
ISSN 0185-6014 (Print)
Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2015, Number 3

<< Back Next >>

Rev Mex Patol Clin Med Lab 2015; 62 (3)

Verification of the biological reference limits of leptin in eutrophic Mexican young women

Castillo-Fregoso MC, Valles-Medina AM, Menchaca-Díaz R, Rosales-Aguilar M, Reyes-Sánchez JD, Magaña-Loaiza C

Language: Spanish
References: 20
Page: 146-149
PDF size: 283.43 Kb.


ABSTRACT

Leptin has been associated with problems of obesity and metabolic disorders such as insulin resistance. The biological reference limits in the Mexican population have been little studied. Objective: To evaluate the consistency and transferability of the biological reference limits of leptin with the ones established by the manufacturer of the reagent and the ones mentioned in the literature. Methods: Cross-sectional study in young Mexican women without overweight or obesity (IMC ‹25). Leptin (ELISA) in serum was estimated by comparing the results with the reference limits marked in the insert and those reported by other authors. The results were analyzed using SPSS v17; Student t and the Pearson correlation coefficient were used. An alpha of 0.05 was considered significant (two tailed). Results: The values of leptin were found to be between 3.6 and 30.8 ng/dL, with an average of 17.8 (± 8.5), confidence interval of 95% for the average of 14.25-21.35 ng/dL. The levels of leptin we registered differ significantly from the ones mentioned in the insert of the reagent (p ≤ 0.001), the Chilean (p = 0.001), Argentinian (p ≤ 0.001), and Colombian (p = 0.004); they were similar to the ones found in Uruguay (p = 0.704). The levels of leptin had significant correlation only with the weight (r = 0.451, p = 0.035), and the IMC (r = 0.583, p = 0.008) in the studied sample. Conclusions: The limits of reference of the insert cannot be transferred to the laboratory. They were different to the ones found in other Latin American countries, though they coincide with the ones in Uruguay. The biological reference limits of leptin correlated positively with the weight and IMC; nevertheless, they must be studied more widely in Mexicans.


REFERENCES

  1. Considine RV. Weight regulation, leptin and growth hormone. Horm Res. 1997; 48 Suppl. 5: 116-121.

  2. Arslanian S, Suprasongsin C, Kalhan SC, Drash AL, Brna R, Hanosky JE. Plasma leptin in children: relationship to puberty, gender, body composition, insulin sensitivity, and energy expenditure. Metabolism. 1998; 47 (3): 309-312.

  3. Houseknecht KL, Baile CA, Natteri RL, Spurlock ME. The biology of leptin: a review. J Anim Sci. 1998; 76 (5): 1405-1420.

  4. Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR et al. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med. 1996; 334: 292-295.

  5. Caro JF, Sinha MK, Kolaczynski JW, Zhang PL, Considine RV. Leptin: the tale of an obesity gene. Diabetes. 1996; 45: 1455-1462.

  6. Ghizzoni L, Mastorakos G. Interactions of leptin, GH, and cortisol in normal children. Ann N Y Acad Sci. 2003; 997: 56-63.

  7. Benoit SC, Clegg DJ, Seeley RJ, Woods SC. Insulin and leptin as adiposity signals. Recent Prog Horm Res. 2004; 59: 267-285.

  8. Huang KC, Lin RC, Kormas N, Lee LT, Chen CY, Gill TP et al. Plasma leptin is associated with insulin resistance independent of age, body mass index, fat mass, lipids, and pubertal development in nondiabetic adolescents. Int J Obes Relat Metab Disord. 2004; 28 (4): 470-475.

  9. Steinberger J, Steffen L, Jacobs JR, Moran A, Hong CP, Sinaiko AR. Relation of leptin to insulin resistance syndrome in children. Obes Res. 2003; 11 (9): 1124-1130.

  10. Molero-Conejo E, Morales LM, Fernández V, Raleigh X, Gómez ME, Semprún-Fereira M et al. Lean adolescents with increased risk for metabolic syndrome. Arch Latinoam Nutr. 2003; 53 (1): 39-46.

  11. Gómez L. Niveles de leptina en plasma en una población infantil normal: desde el periodo fetal hasta la adolescencia. Tesis presentada para optar a grado de doctor en medicina. Barcelona: Universidad Autónoma de Barcelona, Facultad de Medicina; 2003.

  12. ALPCO Leptin (Sandwich) ELISA. Enzyme immunoaasay for the quantitive measurement of leptin human serum or plasma. Versión 6.0 07/2008 [acceso 04 de marzo de 2013]. Consultado en: http://www.alpco.com/pdfs/20/20-DR-2395.pdf

  13. International Federation of Clinical Chemistry. Approved recommendation (1988) on the theory of reference values. Part 3. Preparation of individuals and collection of specimens for the production of reference values. J Clin Chem Clin Biochem. 1988; 26: 593-598.

  14. International Federation of Clinical Chemistry. Approved recommendation on the theory of reference values. Part 4. Control of analytical variation in the production, transfer and application of reference values. J Clin Chem Clin Biochem. 1991; 29: 531-535.

  15. Padró MA, Fuentes AX. Lack of randomness of internal quality control data: an alert for the in vitro diagnostic industry. Scand J Clin Lab Med. 2007; 67: 253-255.

  16. Clinical and Laboratory Standards Institute. Defining, establishing, and verifying reference intervals in the clinical laboratory; proposed guideline. CLSI document C28-P3. 3rd edition. Wayne: CLSI; 2008.

  17. Albala BC, Pérez BF, Santos MJL, Yáñez GM, Arroyo AP, Díaz CJ et al. Relación entre leptina e insulina sanguíneas en mujeres chilenas obesas y no obesas. Rev Med Chile. 2000; 128 (2): 154-161.

  18. Molero CE et al. Insulina, leptina y hormona de crecimiento y su relación con índice de masa corporal e índice de obesidad en adolescentes. ALAN. 2006; 56 (1): 29-35. ISSN 0004-0622.

  19. Poveda E, Callas EN, Baracaldo CN, Castillo C, Hernández P. Concentración sérica de leptina en población escolar de cinco departamentos del centro-oriente colombiano y su relación con parámetros antropométricos y perfil lipídico. Biomédica [online]. 2007; 27 (4): 505-514.

  20. Pisabarro R et al. Leptina: una hormona secretada por el tejido adiposo. Primer estudio en muestra poblacional uruguaya. Rev Med Uruguay. 1999; 15: 43-48.




2020     |     www.medigraphic.com