Identification of Actinobacillus pleuropneumoniae biotype 1, serotype 1, from pig lungs with and without pneumonic lesions using an immunohistochemical technique

Rigoberto Hernández-Castro; Gilberto Chávez-Gris; José Ángel Gutiérrez-Pabello

2002, Number 4

Next >>

Vet Mex 2002; 33 (4)

Identification of Actinobacillus pleuropneumoniae biotype 1, serotype 1, from pig lungs with and without pneumonic lesions using an immunohistochemical technique

Hernández-Castro R, Chávez-Gris G, Gutiérrez-Pabello JA

Full text

How to cite this article

Language: English/Spanish
References: 17
Page: 355-362
PDF size: 74.28 Kb.

ABSTRACT

In the present study the avidin-biotin-peroxidase complex was used for the detection of Actinobacillus pleuropneumoniae biotype 1, serotype 1, and results were compared with bacterial isolation. Lungs from 100 clinically healthy pigs were collected at a slaughterhouse, 50 had apparent pneumonic lesions while 50 had no gross lung lesions. All samples were examined by histopathological, bacteriological and immunohistochemical methods. Predominant microscopic lesions were bronchopneumonia (30%) and interstitial pneumonia (20%). The immunohistochemical technique identified the presence of A. pleuropneumoniae in 75 lungs, whereas bacteriology only detected this microorganism in 11 samples. Statistical analysis showed a significant difference (P ‹ 0.001) in the rate of bacterial identification. Immunohistochemistry may be a good alternative in the routine diagnosis of swine pleuropneumonia.

REFERENCES

1. Nicolet J. Actinobacillus pleuropneumoniae. In: Leman AD, Straw B, Mengeling WL, D’Allaire S, Taylor DJ, editors, Diseases of swine. Ames (Io): Iowa State University Press, 1992:401-408.
2. Blackall PJ, Klaasen HL, Van Den Bosch H, Kuhnert P, Frey J. Proposal of a new serovar of Actinobacillus pleuropneumoniae: serovar 15. Vet Microbiol 2002;84:47-52.
3. Bosse JT, Janson H, Sheehan BJ, Beddek AJ, Rycroft AN, Simon Kroll J, Langford PR. Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection. Microbes Infect. 2002;4:225-235.
4. Dom P, Haesebrouck FF. Comparative virulence of NAD-dependent and NAD-independent Actinobacillus pleuropneumoniae strains. J Vet Med B 1992;39:303-306.
5. Macinnes JI, Smart NL. Actinobacillus and Haemophilus. In: Gyles CL, Thoen CV, editors. Pathogenesis of bacterial infections in animals. Ames (Io): Iowa State University Press, 1993:188-200.
6. Dider PJ, Perino L, Urbance J. Porcine Haemophilus pleuropneumoniae: microbiology and pathologic finding. J Am Vet Med Assoc 1984;184:716-719.
7. Chiers K, Haesebrouck F, Van Overbeke I, Charlier G, Ducatelle R. Early in vivo interactions of Actinobacillus pleuropneumoniae with tonsils of pigs. Vet Microbiol 1999;68:301-306.
8. Gutiérrez CB, Tascon RI, García FJ, Vázquez JA, Rodríguez-Ferri E F. Diagnóstico de la pleuropneumonía porcina. Med Vet 1991;8:3-22.
9. Haesebrouck F, Chiers K, Van Overbeke I, Ducatelle R. Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection. Vet Microbiol 1997;58:239-249.
10. Yolken RH, Leister FJ, Whitcomb LS, Santoshaw M. Enzyme immunoassay for the detection of bacterial antigens utilizing biotin-labeled and peroxidase biotin-avidin complex. J Immunol Meth 1983;56:319-327.
11. Ajito T, Haga Y, Homma S, Goryo M, Okada K. Immunohistological evaluation on respiratory lesions of pigs intranasally inoculated with Actinobacillus pleuropneumoniae serotype 1. J Vet Med Sci 1996;58:297-303.
12. Takashima H, Sakai H, Yanai T, Masegi T. Detection of antibodies against Actinobacillus pleuropneumoniae serotype 1, 2, 5 and 7 using the immunohistochemical staining. J Vet Med Sci 1999;61:713-716.
13. Sidibe M, Messier S, Lariviere S, Gottschalk M, Mittal KR. Detection of Actinobacillus pleuropneumoniae in the porcine upper respiratory tract as a complement to serological tests. Can J Vet Res 1993;57(3):204-208.
14. Ibargoyen GS, Perfumo CJ, Massone AR, Martin AA, Gimeno EJ. The use of immunoperoxidase techniques for the identification of Actinobacillus pleuropneumoniae in tissue sections. Isr J Vet Med 1989;45:18-22.
15. Ohishi K, Koeda T, Susuki S, Muramatsu M. Detection of Actinobacillus pleuropneumoniae antigen by immunoperoxidase staining in pulmonary lesions of pigs inoculated with Actinobacillus pleuropneumoniae. Ann Rep Natl Vet Assay Lab 1986;24:21-26.
16. Gutierrez CB, Rodriguez-Barbosa JI, Suarez J, Tascon RI, Rodriguez Ferri EF. Evaluation of an immunoperoxidase technique using only Biotin-labeled antibody for the demonstration of Actinobacillus pleuropneumoniae in tissue sections. J Vet Med B 1993;40:81-86.
17. Fenwick B, Henry S. Porcine pleuropneumonia. J Am Vet Med Assoc 1994;204:1334-1340.