2014, Number 3
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Biotecnol Apl 2014; 31 (3)
Microbiological evaluation and pathogenicity of a liquid bioformulation of the fungus Purpureocillium sp. (strain UdeA 0109) on Meloidogyne incognita-javanica stages
Cardona NL, Borrego DA, Fernández EP, Sánchez J, Cardona V, Montoya G
Language: English
References: 22
Page: 210-215
PDF size: 513.04 Kb.
ABSTRACT
The BIOMA research group (Biocontrol and environmental microbiology) has an industrial liquid formulation based on
Purpureocillium sp. (UdeA 0109 strain), developed with the collaboration of Laverlam S.A, a Colombian (Cali) commercial house. In the present study the researchers tested the viability and the purity at different storage temperatures as well as its biological potential both
in vitro and under greenhouse conditions. The results of viability showed how the infective structures were affected neither by the evaluation time nor by the storage temperatures. Studies also showed that the purity of the bioformulation in the same conditions was over 99 %, and its pathogenicity in vitro with an LC
50 of 10
4 conidia/mL was corroborated. The greenhouse tests showed the ability to produce damages in eggs of the
Meloidogyne incognita-javanica complex, and a decreasing of the J2 stages at a concentration of 10
8 conidia/mL in three tests performed with a different number of applications and at different times each.
REFERENCES
Abad P, Favery B, Rosso MN, Castagnone-Sereno P. Root-knot nematode parasitism and host response: molecular basis of a sophisticated interaction. Mol Plant Pathol. 2003;4(4):217-24.
Batista FA, Alves SB, Alves LFA, Pereira RM, Augusto NT. Formulção de entomopatogenos. In: Controle Microbiano de Insetos. 2 ed. São Paulo: FEALQ; 1998. p. 917-66.
Vélez A, Posada F, Marín P, González G, Osorio VE, Bustillo P. Técnicas para el control de calidad de formulaciones de hongos entomopatógenos. Bol Téc Cenicafé. 1997;17:1-37.
Luangsa-Ard J, Houbraken J, van Doorn T, Hong SB, Borman AM, Hywel-Jones NL, et al. Purpureocillium, a new genus for the medically important Paecilomyces lilacinus. FEMS Microbiol Lett. 2011;321(2):141-9.
Hewlett TE, Dickson DW, Mitchell DJ, Kannwischer-Mitchell ME. Evaluation of Paecilomyces lilacinus as a biocontrol agent of Meloidogyne javanica on Tobacco. J Nematol. 1988;20(4):578-84.
Sánchez JY. Estudio de viabilidad de las estructuras del hongo Purpureocillium sp. cepa UdeA0109 formulado en un ingrediente inerte bajo diferentes condiciones de tiempo y temperatura. Trabajo de grado para optar por el Título de Biólogo. Universidad de Antioquia. Facultad de Ciencias Exactas y Naturales. Seccional oriente - Carmen de Viboral. Grupo BIOMA. Universidad de Antioquia; 2013.
Cardona HV, Montoya TGJ. Evaluación de la patogenicidad del bioformulado de esporas de Purpureocillium spp. (Cepa UdeA 0109) sobre poblaciones de Meloidogyne spp. en plantas de tomate (Solanum lycopersicum) bajo condiciones de invernadero. Trabajo de pregrado para optar por el título de Biólogo. Medellín (Colombia). Grupo BIOMA. Universidad de Antioquia; 2013.
Margesin R, Schinner F. Manual for soil analysis - Monitoring and assessing soil bioremediation. Soil Biology Vol. 5. Berlin: Springer; 2005.
Coyne DL, Nicol JM, Claudius-Cole B. Practical plant nematology: a field and laboratory guide. SP-IPM Secretariat. Cotonou: International Institute of Tropical Agriculture (IITA); 2007.
Bastidas A, Velásquez SE, Marín P, Benavides P, Bustillo AE, Orozco FJ. Evaluación de preformulados de Beauveria bassiana (bálsamo) Vuillemin, para el control de la broca del café. Agron. 2009;17(1):44-61.
Hong TD, Edgington S, Ellis RH, de Muro MA, Moore D. Saturated salt solutions for humidity control and the survival of dry powder and oil formulations of Beauveria bassiana conidia. J Invertebr Pathol. 2005;89(2):136-43.
Gato CY. Métodos de conservación y formulación de Trichoderma harzianum rifai. Fitosanidad. 2010;14(3):189-95.
Urtuvia UH, France A. Formulaciones de hongos entomopatógenos para control de plagas. Inia Tierra Adentro. 2007;(nov.- dic.):46-9.
Alzate D, Guzmán OA, Leguizamón Caycedo J. Efecto in vitro de Purpureocillium lilacinum (Thom) Luangsa, Ard et al. y Pochonia chlamydosporia (Goddard) Zare y Gams sobre el nematodo barrenador Radopholus similis (Cobb) Thorne. Agron. 2012;20(2):25-36.
Anastasiadis IA, Giannakou IO, Prophetou- Athanasiadou DA, Gowen SR. The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes. Crop Protect. 2008;27:352-61.
Kiewnick S, Mendoza A, Sikora RA. Efficacy of Paecilomyces lilacinus strain 251 for biological control of the burrowing nematode Radopholus similis [Abstract]. J Nematol. 2004;36:326-7.
Kiewnick S, Sikora RA. Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biol Control. 2006;38(2):179-87.
López-Llorca LV, Jansson HB. Biodiversidad del suelo: control biológico de nemátodos fitopatógenos por hongos nematófagos. Cuadernos de Biodiversidad. 2001;(6):12-5.
Stirling GR. Biological control of plant parasitic nematodes: soil ecosystem management in sustainable agriculture. 2ed. Wallingford: CABI; 2014.
Sahebani N, Hadavi N. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Soil Biol Biochem. 2008;40(8):2016-20.
Mendoza AR, Sikora RA, Kiewnick S. Influencia de la dosis y el tiempo de aplicación de Paecilomyces lilacinus cepa 251 sobre el control biológico del nematodo barrenador Radopholus similis en banano. Nematropica. 2007;37(2):203-13.
Corporación Colombiana de Investigación agropecuaria CORPOICA. Listado de empresas de bioinsumos registradas. 2013 [cited 2013 Oct 2]. Available from: http://www.ica.gov.co/Areas/Agricola/ Servicios/Fertilizantes-y-Bio-insumos- Agricolas/Listado-de-Bioinsumos/2009/ EMPRESAS-REGISTRADAS-BIOINSUMOSJULIO- 8-DE-2008.aspx.