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2014, Número 3

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Biotecnol Apl 2014; 31 (3)


Determinación del efecto del tamaño y la edad del inóculo en la producción de lipasa por Geotrichum candidum

Resende MR, Fernandes MBJ, Aguiar-Oliveira E, Durrant L, Mazutti MA, Maugeri FF, Rodrigues MI

Idioma: Ingles.
Referencias bibliográficas: 36
Paginas: 216-221
Archivo PDF: 293.59 Kb.


RESUMEN

Las lipasas son enzimas extremadamente versátiles. Se utilizan en varias aplicaciones industriales, y han sido objeto de atención recientemente. Una diversidad de microrganismos puede producirlas, tales como los hongos pluricelulares. Sin embargo, para el estudio de estas enzimas se debe optimizar su crecimiento, debido a las variaciones entre los experimentos, que influyen en los resultados y en la reproducibilidad de los procesos. El objetivo de este trabajo fue investigar el tamaño y la edad idóneos del inóculo Geotrichum candidum NRRLY-552, con el fin de reducir su variabilidad. El procedimiento optimizado para el inóculo se determinó como una zona circular (0.79 cm2) de medio sólido, que contenía células y esporas, añadido a 100 mL de medio de cultivo (peptona 5.0 % p/v, NaNO3 0.1 % p/v, MgSO4 0.1 % p/v y aceite de soya 1.0 % p/v) que se incubaron durante 15 h a 30 °C, 250 rpm, con un pH inicial de 7.0. Este procedimiento permitió reducir el error experimental de 30 a 20 %, y se ha aplicado con éxito en varios estudios.


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