2004, Number 5
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Gac Med Mex 2004; 140 (5)
Isolation and Characterization of Wild Sporothrix schenkii Strains and Investigation of Sporototrichin Reactors.
Sánchez-Alemán MA, Araiza J, Bonifaz A
Language: Spanish
References: 26
Page: 507-512
PDF size: 510.73 Kb.
ABSTRACT
We conducted a study in the southern mountains of the
Mexican State of Oaxaca that consisted of isolation of wild
Sporothrix schenckii strains obtained from soil samples
and investigation of positive reactors to skin test reaction
with sprotrichin antigen. The study was conducted by
means of recollection of soil samples and processing of
these with dilution methods and fungal isolation in ordinary
culture media Sabouraud simple Agar with and without
antibiotics (SS, SA). Suspected strains underwent dimorfism,
melanin formation, and virulence confirmation tests.
Investigation of positive reactors to sporotrichin Y (yeast)
was also conducted. Three supposed strains were identified
due to their reproductive characteristics, melanin production,
and virulence. In the community; 144 individuals were
studied, of whom 6.25% were positive to sporotichin. Isolation
of virulent strains of Sporotrhix schenkii from nature (soil)
and primoinfection of a percentage of the studied population
were confirmed.
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