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ISSN 0210-5187 (Electronic)

2003, Number 4

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Med Cutan Iber Lat Am 2003; 31 (4)

Quantitative Immunohistochemical Study of Proliferation Index and Apoptosis Index in Acné Vulgaris

Vignale R, Reissenweber N

Language: Spanish
References: 22
Page: 238-242
PDF size: 304.41 Kb.


ABSTRACT

Acné vulgaris is a multifactorial common disease involving the pilosebaceous follicle. Hyperkeratinization in the infrainfundibulum constitutes the primary element in he formation of comedones. We studied skin biopsies of 20 patients with acné vulgaris with closed comedo or microcystic (Whitehead) for immunohistohemistry for determination of the Proliferation Index with PCNA and the apoptosis Index with TUNEL tecniques. We compared with 5 biopsies with normal skin without acné for control. Only we studied this types comedones, not the open comedones (Blackhead) because they have different clinical and histophatological features. We studied three different areas of the epithelium of the pilosebaceous unit: epidermis of the surface close to the ostium, in the infrainfundibulum and the sebaceuos duct. The results showed that both índices were significantly increased evaluated at the epithelium infundibular and the excreting duct of the sebaceous gland compared to the epidermis close to the ostium. We suggest that in cystic or close comedo exhibit a greater production and mitotic activity and a greater rate of cell apoptosis leading to an increased in desquamation “ hyperkeratosis” in all the pilosebaceous follicle than in acné free. This means that the infrainfundibulum, the duct sebaceous and the gland operate and responde as a similar unit to form this types of comedo. It is very important the participation of the duct to produce the hyperkeratinization in the firsts stages of this close comedo as in the infrainfundibulum. For the contrary, this event not succeed in the first etages in the open comedo.


REFERENCES

  1. Kligman AM. An overview of acné. J Invest Dermatol 1974; 62: 268-87.

  2. Knutson DD. Ultrastructural observations in acné vulgaris: the normal sebaceous follicle and acné lesions. J Invest Dermatol 1974;62: 288-307.

  3. Shalita AR. Acné vulgaris. Current Concept in Pathogenesis and Treatment. Int J Dermatol. 1976; 15: 182-87.

  4. Plewig G. Acné vulgaris: proliferative cells in sebaceous gland. Br J Dermatol 1974; 90: 625–30.

  5. Plewig G. Morphologic dynamics of acné vulgaris. Acta Dermatovener (Stockholm) Suppl 89; 1980: 9-16.

  6. Plewig G, Kligman AM. Sebaceous Glands. En: Acné and Rosacea. eds. 2nd. Springer-Verlag. Berlin. 1993: 33-37.

  7. Leyden JJ. New undertanding of the pilosebaceous of acné. J Am Acad Dermatol1995; 32: S15 – S25.

  8. Janssen T, Plewig G, Kligman AM. Pathophysiology of acné. Dermatologic Therapy 1998; 6: 7-17.

  9. Knaggs HE, Holland DB, Morris C, Wood ES, Cunliffe WJ. Quantification of cellular proliferation in acné using the monoclonal antibody Ki-67. J Invest Dermatol 1994; 102: 89- 92.

  10. Hughes BR, Morris C, Cunliffe WJ, Leigh IM. Keratin expression pilosebaceous epithelia in truncal skin of acné patients. Br J Dermatol 1996; 134: 247 – 56.

  11. Cunliffe WJ, Holland DB, Clark SM, Stables GI. Comedogenesis: some new aetiological, clinical and therapeutic strategies. Br J Dermatol 2000;142: 1084 – 1091.

  12. Guy R, Kealey T. Modelling the infundibulum in acné. Dermatology 1998; 196; 32-7.

  13. Van Scott EJ, MacCardle RC. Keratinization of the duct of the sebaceous gland and growth cycle of the hair follicle in the histogenesis of acné in human skin. J Invest Dermatol 1956; 27: 405 – 29.

  14. González-Serva A. Sebolemmal keratininization: origin in the sebaceous gland duct of the cornified casing of the sebum plug. J Cutan Pathol (Abstract).1989; 16: 305.

  15. González-Serva A The sebaceous gland duct as the source of the sebolemmal keratinous casing of the sebum plug. J Invest Dermatol (Abstract). 1990; 94: 530.

  16. González-Serva A. The sebolith: an aneigenic follicular concretion (Abstract) J Cutan Pathol 1992; 19: 525.

  17. González-Serva A. La patogenesis del acné: de un paradigma hiperqueratosico (comedon) a uno de calculosis folicular (sebolito). Med Cutan Ibero Lat Am 1996; 24: 11-25.

  18. Sánchez Yus E. La unidad folículo-sebáceo-apócrina y sus quistes. Curso de Dermatopatología. Madrid. España. 1994

  19. Simón RS, De Eusebio E, Alvarez-Vietez A, Sánchez-Yus E. Proliferaciones con diferención sebácea. I: Hamartomas. Actas Dermo- Sif 1998; 89: 577-90.

  20. Borbujo Martinez JM. Etiopatogenia del Acné Vulgar. Monografias de Dermatologia. Acné I. Soto Melo J ed.. España. 1990,III. 18-25.

  21. Vignale R, Panuncio A. Clasificación histopatológica de los comedones en el Acné vulgaris. En prensa en Dermatología Argentina 2003.

  22. Gabrieli Y, Ben-Sasson SA. J Cell Biol 1992; 119: 493-501.




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