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2014, Number 3

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Rev Cubana Farm 2014; 48 (3)

Validation of analytical method for dopamine determination by high-performance liquid chromatography

Hernández FD, Fernández GA, Ledea LOE

Language: Spanish
References: 17
Page: 371-381
PDF size: 280.74 Kb.


ABSTRACT

Introduction: dopamine is an endogenous hormone in the catecholamine group, which is used to simulate the sympathetic nervous system action and to raise the heart rate and the blood pressure. However, this drug has high genotoxicity and is associated with the treatment of diseases such as schizophrenia and Parkinson's disease. For this reason, the determination of dopamine in wastewaters coming from its production has been of great interest.
Objective: to validate a high performance liquid chromatography method for quantification of dopamine present in aqueous solutions.
Methods: the validation used a RP-18 250 × 4.6 mm 5 µm column, mobile phase: 1 % NaH2PO4 /CH3OH (90/10), 1.0 mL/min flow rate and a visible ultraviolet detector at 280 nm. Detection limits and quantitation were estimated in addition to evaluating the drug stability under the analysis conditions.
Results: documented evidence showed that the method proved to be linear (r= 0.999 and r2= 0.998), accurate and precise in the range of 1 × 10-4 mol/L to 2 × 10-3 mol/L. This method was also selective against potential degradation products from ozonization.
Conclusions: the studied method was linear, precise and accurate in the range of tested concentrations, allowing the dopamine determination in aqueous solutions.


REFERENCES

  1. Bahena R, Flores G, Arias JA. Dopamina: síntesis, liberación y receptores en el Sistema Nervioso Central. Rev Biomed. 2000;11(1):39-60.

  2. Hornykiewicz O. Dopamine (3-hidroxytyramine) and brain function. Pharmacol Rev. 1996;18:925-64.

  3. Meana JJ, García JA. Transmisión catecolaminérgica. Fármacos agonistas catecolaminérgicos. En: Flores J. Farmacología Humana. 5ta ed. Espańa: Editoral Elsevier; 2008. p. 235-60.

  4. Stokes AH, Hastings TG, Vrana KE. Cytotoxic and genotoxic potential of dopamine. Mini-Review J Neurosci Res. 1999;55:659-65.

  5. Jing L, Haiyan D, Wanzhi W, Shenglian L. Spectrometric investigations on the binding of dopamine to bovine serum albumin. Physics and Chemistry of Liquids. 2012;50(4):453-64.

  6. Sopis Ch, Sukon P, Suwan Ch. Fabrication of Modified SWNTs/Glassy Carbon Electrode for the determination of dopamine. Molecular Crystals and Liquid Crystals. 2011;538(1):292-7.

  7. Yar M, Ali L, Ali S, Ali A. Capillary gas chromatographic determination of dopamine by pre-column derivatization method using trifluoroacetylacetone as Derivatizing reagent. J Chem Soc Pak. 2010;32(6):781-5.

  8. Musshoff F, Schmidt P, Dettmeyer R, Priemerb F, Jachauc K, Madeaa B. Determination of dopamine and dopamine-derived (R)-/(S)-salsolinol and norsalsolinol in various human brain areas using solid-phase extraction and gas chromatography/ mass spectrometry. Forensic Science International. 2000;113:359-66.

  9. Syslová K, Rambousek L, Kuzma M, Najmanová V, Bubeníková V, Slamberová R, et al. Monitoring of dopamine and its metabolites in brain microdialysates: method combining freeze-drying with liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2011;1218(21):3382-91.

  10. Głód BK, Staczak KI. Application of RP-HPLC-ED assay to analysis of the blood of Parkinson's disease patients. Acta Chromatog. 2005;15:276-88.

  11. El-Beqqali A, Kussak A, Abdel M. Determination of dopamine and serotonin in human urine samples utilizing microextraction online with liquid chromatography/electrospray tandem mass. Spectrometry. J Sep Sci. 2007;30:421-4.

  12. Wang Y, Zhang X, Chen Y, Xu H, Tan Y, Wang S. Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor. Am J Biomed Sci. 2010;2(3):209-16.

  13. ICH-Harmonised tripartite Guideline. Validation of analytical Procedures: Text and Methodology Q2(R1). 2005.

  14. WHO-Technical Report Series No. 937. Appendix 4n Analytical Method Validation: 2006.

  15. EURACHEM Guide: The Fitness for Purpose of Analytical Methods. A Laboratory Guide to Method Validation and Related Topics. 1998.

  16. Bonfilio R, Laignier EC, Benjamim M, Nunes HR. Analytical Validation of Quantitative High-Performance Liquid Chromatographic Methods in Pharmaceutical. Analysis: A Practical Approach. Crit Rev Analyt Chem. 2012;42(1):87-100.

  17. Kazusaki M, Ueda S, Takeuchi N, Ohgami Y. Validation of analytical procedures by high−performance liquid chromatography for pharmaceutical analysis. Chromatography. 2012;33:(2):65-73.




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