Meneses GAP, França OJF

Language: Portugués
References: 26
Page: 189-198
PDF size: 110.29 Kb.

ABSTRACT

Introduction: natural products obtained from plants play an important role in the process of discovery of new drugs, either as structural models for the synthesis of new molecules or due to their pharmacological properties. A widely used medicinal plant is Mangifera indica Linneau, of the family Anacardiaceae. Popularly known as 'mango', it is used to treat infection, though its effect has not been scientifically validated.
Objective: evaluate the phytochemical composition and antimicrobial activity in vitro of the hydroalcoholic extract from Mangifera indica L. using the disk diffusion method.
Methods: the crude extract was prepared from dry leaves macerated for 15 days at a proportion of 30 g to 300 ml of 95 % ethanol. After filtration, the extract was concentrated and dried to obtain the crude ethanol extract. Antimicrobial activity was determined by the disc diffusion method on Mueller-Hinton agar medium, in compliance with Standard M07-A8. Tests were performed in triplicate and results were expressed in millimeters (mm). The diameter of inhibition areas around the discs is formed in three replicates.
Results: results show that the crude hydroalcoholic extract from M. indica had antimicrobial activity in vitro against the S. aureus strain with a minimum inhibitory concentration (MIC) of 1.0 µg.disc-1. No antimicrobial activity was observed against gram-negative bacteria (E. coli, P. aeruginosa and S. typhi).
Conclusion: the crude hydroalcoholic extract from Mangifera indica L. displayed antimicrobial activity against Staphylococcus aureus, providing scientific evidence for its potential therapeutic use.

REFERENCES

1. Oliveira CJ, Araújo TL. Plantas medicinais: usos e crenças de idosos portadores de hipertensão arterial. Revista Eletrônica de Enfermagem. 2007;9(1):93-105.

2. Silveira LMS, Olea RSG, Mesquita JS, Cruz ALN, Mendes JC. Metodologias de atividade antimicrobiana aplicadas a extratos de plantas: comparação entre duas técnicas de ágar difusão. Revista Brasileira de Farmácia. 2009;90(2):124-128.

3. Salvagnini LE, Oliveira JRS, Santos LE, Moreira RR, Pietro RCLR. Avaliação da atividade antibacteriana de folhas de Myrtus communis L. (Myrtaceae). Revista Brasileira de Farmacognosia, Brazilian Journal of Pharmacognosy. 2008;18(2):241-244.

4. Pitchaon M. Antioxidant capacity of extracts and fractions from mango (Mangifera indica Linn.) seed kernels. International Food Research Journal. 2011;18(1):523-528.

5. Braga R. Plantas do nordeste - Especialmente do Ceará. 5a ed. Ceará, Brasil: Coleção Mossoroense; 2001.p. 204

6. Costa CTC, Morais SM, Bevilaqua CML, Souza MMC, Leite FKA. Efeito ovicida de extratos de sementes de Mangifera indica L. sobre Haemonchus contortus. Revista Brasileira de Parasitologia Veterinária. 2002;11(2):57-60.

7. Ferreira FS, Santos SC, Barros TF, Rossi-Alva JC, Fernandez LG. Atividade antibacteriana in vitro de extratos de Rhizophora mangle L. Rev. Bras. Plantas Med. 2011;13(3):305-310.

8. Bispo NJ, Francisco NMAC, Schimitt AC. Avaliação da atividade antimicrobiana in vitro dos extratos da planta Guettarda angelica sobre bactérias Gram-positivas e Gram-negativas. LAES & HAES. 2007;168(1):165-169.

9. Silva GJ, Souza IA, Higino JS, Siqueira-Junior JP, Pereira JV, Pereira MV. Atividade antimicrobiana do extrato de Anacardium occidentale Linn. em amostras multiresistentes de Staphylococcus aureus. Revista Brasileira de Farmacognosia. 2007;17(4):572-577.

10. Santos SC, Ferreira FS, Damião AO, Damião AO, Barros TF, Rossi-Alva JC, et al. Avaliação da atividade antibacteriana dos extratos de Avicennia schaueriana Stapf & Leechm. Ex Moldenke, Verbenaceae. Revista Brasileira de Farmacognosia. 2010;20(1):124-129.

11. Braga FG, Bouzada MLM, Fabri RL, Matos MO, Moreira FO, Scio E, et al. Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil. Journal of Ethnopharmacology. 2007;111(2):396-402.

12. Barbosa WLR, Quinard E, Tavares ICC, Pinto LN, Oliveira FQ, Oliveira RM. Manual para análise fitoquímica e cromatográfica de extratos vegetais. 2 ed. Revista Científica da UFPA, 2004.p. 19.

13. Matos FJA. Introdução a fitoquímica experimental. 2ª ed. Fortaleza, Brasil: UFC:1997. p. 141.

14. CLINICAL AND LABORATORY STANDARDS INSTITUTE (CLSI). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard. 4ª ed. Wayne, Pennsylvania: CLSI. 2009. p. 99.

15. Santos SC, Ferreira FS, Rossi-Alva JC, Fernandez LG. Atividade antimicrobiana in vitro do extrato de Abarema cochliocarpos (Gomes) Barneby & Grimes. Revista Brasileira de Farmacognosia. 2007;17(2):215-2159.

16. Carvalho AAT, Sampaio MCC, Sampaio FC, Melo AFM, Sena KXFR, Chiappeta AA, et al. Atividade antimicrobiana in vitro de extratos hidroalcoólicos de Psidium guajava L. sobre bactérias gram-negativas. Acta Farmacêutica Bonaerense. 2002;21(4):255-258.

17. Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV Dias Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 2002;97(7):1027-1031.

18. Parekh J, Chanda SV. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J. Biol. 2007;31(1):53-58.

19. França HS, Kuster RM, Rito PN, Oliveira AP, Teixeira LA, Rocha L. Atividade antibacteriana de floroglucinóis e de extrato hexânico de Hypericum brasiliense Choysi. Quim. Nova. 2009;32(5):1103-1106.

20. Reichling J, Koch C, Stahl-Biskup E, Sojka C, Schnitzler P. Virucidal activity of a beta-triketone-rich essential oil of Leptospermum scoparium (manuka oil) against HSV-1 and HSV-2 in cell culture. Planta Med. 2005;71(1):1123–1127.

21. Cox SD, Mann CM, Markham JL, Bell HC, Gustafson JE, Warmington JR, et al. The mode of antimicrobial action of essential oil of Melaleuca alternifolia (tea tree oil). Journal Applied. Microbiology. 2000;88(1):170-175.

22. Botsaris AS. Fitoterapia chinesa e plantas brasileiras. São Paulo; Ícone, 2007. p.550

23. Almassy Júnior AA. Folhas de Chá: plantas medicinais na terapêutica humana. Viçosa: UFV, 2005. p 233.

24. Laurens A, Giono B, Sylla DP. Etude de l'action antibacterenne d'extraits d'Anacardium occidentale L. Annales Pharmaceutiques Françaises. 1992;40(1):143-146.

25. Melo AFM, Santos EJV, Souza LFC, Carvalho AAT, Pereira MSV, Higino JS. Atividade antimicrobiana in vitro do extrato de Anacardium occidentale L. sobre espécies de Streptococcus. Revista Brasileira de Farmacognosia. 2006;16(2):202-205.

26. Macedo-Costa MRM, Diniz DN, Carvalho CMV. Eficácia do extrato de Myrciaria cauliflora (Mart.) O. Berg. (Jabuticabeira) sobre bactérias orais. Revista Brasileira de Farmacognosia. 2009;19(2):565-571.