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2014, Number 3

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Rev Cubana Plant Med 2014; 19 (3)

Antibacterial and modulatory activity of Cecropia pachystachya Trécul on the action of aminoglycosides

Oliveira SD, Relison TS, Gomes FF, Melo BMC, Bezerra MBMF, Bezerra FCF, Martins CJG, Melo CHD, Alencar MIR, Kerntopf MR

Language: Portugués
References: 45
Page: 121-132
PDF size: 124.39 Kb.


ABSTRACT

Introduction: Cecropia pachystachya Trécul, commonly known as 'toré' (Urticaceae) and distributed throughout Brazil, is used in folk medicine to treat respiratory and sexually transmitted infections such as gonorrhea.
Objective: evaluate the antibacterial and modulatory activity on antibiotics of the ethanolic extract and methanolic fractions of C. pachystachya T. leaves and stems against standard and multiresistant bacterial strains.
Methods: the plant material (leaves and stems) was collected in Araripe, crushed and put into ethanol PA as solvent. Distillation of the solvent was then performed in a rotary evaporator to produce the extracts, which were divided and subjected to the methanolic solvent to obtain the fractions. A microdilution assay was conducted to verify antibacterial activity and the possible interactions of aminoglycosides associated with the study samples. A sub-inhibitory concentration of 128 µg/mL (MIC/8) was used.
Results: the most representative effect was the association of neomycin with EEFCP (ethanolic extract of leaves of C. pachystachya T.) and its fraction, with a MIC reduction of 312.5 for 39.06 µg/mL and 312.50 for 78.13 µg/mL, respectively, against Staphylococcus aureus 358. An antagonistic effect was observed against E. coli 27.
Conclusions: the data obtained indicate that C. pachystachya methanolic fractions and extract may be an important alternative in the search for substances with an action against multiresistant Staphylococcus bacteria. However, such a substance may also negatively affect the action of the drugs studied. It is necessary to conduct new experiments in vivo to verify such an action.


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