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ISSN 0185-6014 (Print)
Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2015, Number 1

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Rev Mex Patol Clin Med Lab 2015; 62 (1)

Detection of human papilloma virus in self-collected vaginal swabs

Narváez L, Loayza F, Narváez M, Vega X, Vargas P, Sáenz K

Language: Spanish
References: 24
Page: 5-10
PDF size: 296.43 Kb.


ABSTRACT

An alternative screening strategy using self-collected vaginal swabs was evaluated and compared with conventional physician-collected cervical samples for molecular diagnosis of infection with human papillomavirus. An uncontrolled clinical trial was performed in self- and physician-collected samples from 90 women that were attending the Isidro Ayora Gyneco-obstetric Hospital (Quito, Ecuador). Each patient completed a form of demographic, clinical and sexual behavior information. Then patients were trained for self-collection of a vaginal swab, and subsequently, physicians collected conventional cervical samples for detection of viral infection (Amplicor®-Roche Diagnostics). The mean age was 37.14 ± 8.07 years. The overall prevalence of viral infection in cervical samples was 24.44% (CI95% 15.6-33.3%) and 27.78% (CI95% 18.5-37.0%) in self-collected swabs (p › 0.05). Sensitivity of self- collected samples was 90.9%, with a specificity of 93%, the positive and negative likelihood radio was 12.8 and 0.1 respectively, with a total agreement of 92% (κ = 0.799) between results. In conclusion, self-sampled vaginal swabs collected by patients are a valid alternative to facilitate the access and compliance of patients to perform studies of molecular biology as a primary test for the screening of high-risk papillomavirus infection.


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