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2012, Number 1

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Dermatología Cosmética, Médica y Quirúrgica 2012; 10 (1)

Comparison between histopathology and PCR, for the diagnosis of tegumentary Leishmaniasis

Ríos YJM, Saldaña A, Calzada J, Arias J, Díaz R, González K

Language: Spanish
References: 37
Page: 13-20
PDF size: 148.41 Kb.


ABSTRACT

Background: Leishmaniasis is caused by parasites of the genus Leishmania. Two million people worldwide are newly infected every year, and unfortunately there isn’t a diagnostic test that serves as a gold standard yet. The objective of this study was to determine which diagnostic technique for this entity is best. We compared the histopathology and the PCR of paraffin embedded biopsies, from the Pathology services of the Complejo Hospitalario Dr. Arnulfo Arias Madrid and the Hospital Santo Tomás of Panama, from January 2007 to December 2010.
Material and method: Analytical, observational, correlational and retrospective trial with paraffin embedded biopsies with clinical diagnosis of tegumentary leishmaniasis. The DNA was extracted with the QIAamp® DNA Mini Kit (Qiagen, Hilden, Germany), and the PCR was performed with a Promega kit (Madison, Wisconsin, USA). The percentage of positives with histopathology and with PCR was determined. Both tests were compared taking proportions into account, and also using the Cohen’s Kappa index.
Results: 50% of the patients were female. The mean age of the patients was 40.7 years, and 50% of them lived in the Panama Province. The histopathology was positive in 38.10%, and the PCR in 30.43% of the paraffin embedded biopsies.
Conclusions: There was no statistical difference, between the positivity percentage of the histopathology and the PCR of the paraffin embedded tissue. Therefore, we cannot consider one technique better than the other. There was no correlation between techniques. Nevertheless, the histopathology is still a routine and a less expensive procedure


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