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2014, Number 2

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Rev Mex Med Repro 2014; 6.7 (2)

A pilot study to determine the levels of superoxide anion (O2-) in semen and its correlation to spermatobioscopy parameters

Góngora-Rodríguez A, Gosálvez J, González-Cortés C, Capilla-González G, Parra-Forero LY

Language: Spanish
References: 46
Page: 75-82
PDF size: 576.34 Kb.


ABSTRACT

Background: During several years the incidence of reactive oxygen species (ROS) on sperm quality has been studied; it is necessary a sinergism between them to determinant processes, such as fecundation and sperm capacitation.
Objective: To assess the application of Kit Oxisperm® on samples of ejaculation of patients and donors of an assisted reproduction center and to correlate their results with parameters of basic spermatobioscopy.
Material and method: An observational and descriptive study was done in which the Kit Oxisperm® was applied based on the colorimetric reaction of blue of nitritotetrazolio (NBT), on 100 samples from ejaculations chosen randomly from donors and patients from a private center of reproduction, they were evaluated according to the parameters of the World Health Organizations (2010). Data were analyzed with SPSS 11.0.
Results: There were significant differences (p‹0.01). Levels of concentrations varied among the samples performed: N1 34%, N2 36% and N3 30%. There was a negative correlation between the motility and level of superoxide anion (r=-0.465, p=0.0007).
Conclusions: The Kit Oxisperm® showed differences in the level of superoxide anion and had a correlation with parameters related with the quality of ejaculation. More studies with a bigger population are needed, because near 64% of samples belonged to fertile patients without evident sperm disorders. The Kit Oxisperm® is a quick and simple test that may be done in any laboratory of assisted reproduction.


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