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2014, Number 1

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Cuba y Salud 2014; 9 (1)

Characterization of Neisseria menigitidis strains causing invasive diseases in Cuba

Suárez NN, Martínez MI, Feliano SO, Gutiérrez O, Valdés HMJ, Villasusa PI, Llanes CR

Language: Spanish
References: 30
Page: 13-19
PDF size: 315.41 Kb.


ABSTRACT

Objective: To characterize the strains of Neisseria menigitidis insulated from invasive processes in Cuba, for the period of 10 years.
Method: Transverse descriptive study of the insulation of N. meningitidis referred to the National Reference Laboratory of Pathogen Neisseriasof “Pedro Kurí” institute from 2002 to 2011. 68 of the 136 strains collected were feasible. The identification and serogrouping was made by conventional methods. When suspicious, the serogroup was confirmed through chain reaction of polymerase. The serosubtypes were identified by the ELISA method of whole cells with monoclonal antibodies. The antimicrobial susceptibility was performed by E-test.
Results: The serogroup B(98,6%) predominated, an insulation was C (1,4%). Serotypes 4 (55,1%) prevailed. The untyping strains (15,9%) and the 2ª (8,7%). The most frequent subtypes were: P1.15 (42,0%), P1.19 (20,3%) and the unsubtyping strains (10,1%). The prevalent phenotype was B: 4:P1.15 (26,1%) and the association C:2ª:P1.5 (1,4%)was detected for the first time in Cuba. The sensitive strains prevailed over the prove antimicrobials, except for the cotrimozaxol whose resistance was high (92, 8%).Also resistance to the ciprofloxacin was found (10, 7%), the penicillin (8,9%) and the chloramphenicol (5,3%).
Conclusions: The phenotype B: 4:P1.15 of Neisseria meningitiss predominated confirming its persistence in Cuba. The phenotype C: 2ª:P1.5 detection for the first time supports the need of maintaining the characterization of the strains as an alert system facing the increase of a given antigenic profile. The prevalence of strains sensitive to penicillin suggests this medicine validity for the treatment of meningococcus in Cuba, although detecting resistant strains to it and to other antimicrobials emphasize the necessity to continue surveillance on this respect.


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