2014, Number 5
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Rev ADM 2014; 71 (5)
Detection of Legionella pneumophila in the water supply systems at the Autonomous University of the State of Mexico’s (UAEM) Faculty of Dentistry
Manzanares LGL, Montiel BNM, del Real SSGME, Flores CRI
Language: Spanish
References: 14
Page: 216-220
PDF size: 209.75 Kb.
ABSTRACT
Background: Water supply systems, including those of dental units, are prone to the development of biofilms that may be colonized by opportunistic pathogens such as
Legionella pneumophila, a type of bacteria that can cause severe lung diseases.
Objective: To determine, for the first time, whether
Legionella pneumophila is present in the water supply systems of the Faculty of Dentistry at the UAEM.
Material and methods: An observational, cross-sectional study was performed in which 120 water samples were collected from the Faculty of Dentistry of the UAEM, including from the water supply lines of dental units, the filters, and the publically-used faucets. These were then cultivated in BCYEα agar with L-cysteine and verified using blood agar. CFU/mL were counted and an ANOVA test and a Bonferroni test were applied with the help of SPSS.21 software package for statistical analysis.
Results: 100% of the samples displayed growth in BCYEα agar and an absence in blood agar. There were statistically significant differences (p ≤ 0.05) in all of the tests.
Conclusions: Six-monthly monitoring is recommended in order to allow timely corrections to be made and to avoid any increase in the bacteria and the development of disease.
REFERENCES
Barbeau J, Gauthier C, Payment P. Biofilms, infectious agents, and dental unit waterlines: a review. Can J Microbiol. 1998; 44: 1019-1028. doi: 10.1139/w98-101.
Bonadonna L, Briancesco R, Della Libera S, Lacchetti I, Paradiso R, Semproni M. Microbial characterization of water and biofilms in drinking water distribution systems at sport facilities. Cent Eur J Public Health. 2009; 17 (2): 99-102.
Prescott LM, Halley JP, Klein DA. Microbiología. 4a ed. Madrid: McGraw Hill Interamericana; 2004.
Craun GF, Brunkard JM et al. Causes of outbreaks associated with drinking water in the United States from 1971 to 2006. Clin Microbiol Rev. 2010; 23 (3): 507-28.
Secretaría de Salud, Sistema Nacional de Vigilancia Epidemiológica, Vigilancia Epidemiológica. 2005; 22 (8).
Koneman EW, Allen S. Diagnóstico microbiológico/microbiological diagnosis: Texto y atlas. 6ª edición. Madrid, España: Editorial Médica Panamericana; 2006.
Atlas RM, Williams JF, Huntington MK. Legionella contamination of dental-unit waters. Appl Environ Microbiol. 1995; 61: 1208-1213.
Ma’ayeh SY, Al-Hiyasat AS, Hindiyeh MY, Khader YS. Legionella pneumophila contamination of a dental unit water line system in a dental teaching centre. Int J Dent Hyg. 2008; 6: 48-55. doi: 10.1111/j.1601-5037.2007.00280.x.
Szymanska J. Risk of exposure to Legionella in dental practice. Ann Agric Environ Med. 2004; 11: 9-12.
Rodríguez E, Gamboa MM, Arias ML. Legionella spp. ¿Ausente en los hospitales de Costa Rica? Rev Biomed. 2002; 13: 165-169.
Exner M, Kramer A, Lajoie L et al. Prevention and control of health care associated waterborne infections in health care facilities. Am J Infect Control. 2005; 33: S26-S40.
Kusnetsov J, Torvinen E, Perola O et al. Colonization of hospital water systems by Legionella, mycobacteria and other heterotrophic bacteria potentially hazardous to risk group patients. APMIS. 2003; 111: 546-556.
Williams JF, Johnston AM, Johnson B, Huntington MK, Mackenzie CD. Microbial contamination of dental unit waterlines: prevalence, intensity, and microbial characteristics. J Am Dent Assoc. 1993; 124: 59-65.
Real Decreto 865/2003, de 4.7 (M. San. y Cons., BOE 18.7.2003), por el que se establecen los criterios higiénico-sanitarios para la prevención y el control de la legionelosis.