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2014, Number 3

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Enf Infec Microbiol 2014; 34 (3)

Extended-spectrum beta-lactamaseproducing Klebsiella pneumoniae and Escherichia coli isolated from patients with health care-associated infection in a university hospital

Abreu S, Varela Y, Millán B, Araque M

Language: Spanish
References: 26
Page: 92-99
PDF size: 184.17 Kb.


ABSTRACT

background. Health care-associated infection (HCAI) caused by extended-spectrum beta-lactamase- (ESBL) producing enterobacteria, represent an emerging public health problem. In this study we determined the presence and distribution of blaESBL genes in K. pneumoniae and E. coli strains isolated from patients with HCAI at The Andes University Hospital (TAUH), Mérida, Venezuela.
materials and methods. Fourteen K. pneumoniae and 12 E. coli strains isolated from patients with HCAI in TAUH collected from March to July 2013 were studied. Susceptibility tests were performed by minimum inhibitory concentration and the presence of ESBLs was detected by the double-disk synergy test. Transfer of ESBLs determinants was performed by conjugation and characterization of the blaESBL genes by PCR.
results. Pneumonia was the most frequent diagnosis of HCAI (30.8%). All strains were ESBL producers, most associated with resistance to quinolones and aminoglycosides. All ESBL determinants were transferred by conjugation. The blaCTX-M-1 gene was the most prevalent in K. pneumoniae (50%), and blaCTX-M-8 in E. coli (50%). The association of different blaESBL genes was observed in 46.1% of strains.
conclusion. To our knowledge, this is the first description of blaCTX-M-8 gene in E. coli associated with blaTEM and blaCTX-M-9 genes in Venezuela.


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