Valdés DS, González SR, Gutiérrez CR, Rosas CA, Romo LY

Language: Spanish
References: 20
Page: 45-50
PDF size: 555.67 Kb.

ABSTRACT

Background: Helicobacter pylori bacterium has been detected in extragastric regions such as oral cavity and middle ear. Some authors have shown evidence that it could also be present in tonsillar tissue,but there is contradictory data about its presence in this site. Bacterial presence in adenotonsillar tissue could be an extragastric reservoir for its transmission and/or reinfection.
Patients and method: An observational, cross-sectional and prospective study was done including 50 patients (7.4 ± 2.9 years) scheduled for tonsillectomy for recurrent pharyngotonsillitis at the Hospital Miguel Hidalgo of Aguascalientes, Mexico, in 2012. Rapid urease test (RUT) was performed from tonsillar tissue in urea buffer. Tonsillar tissue was seeded in blood agar base and incubated at 37^oC in microaerophilic conditions. Genomic DNA was used in PCR obtained from tonsillar tissue for amplification of the segments corresponding to ureC and hsp60 specific bacterial genes.
Results: All samples were negative to RUT. Bacterial culture was negative in all tonsillar tissue samples. PCR amplification of ureC and hsp60 was not identified in any of the patient samples.
Conclusion: The presence of H. pylori was not demonstrated in tonsils of recurrent pharyngotonsillitis patients using three different methods.

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