medigraphic.com
ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2013, Number 4

<< Back Next >>

Biotecnol Apl 2013; 30 (4)

Impact of native phosphate solubilizing bacteria on the growth and development of radish (Raphanus sativus L.) plants

Lara C, Sanes SC, Oviedo LE

Language: Spanish
References: 40
Page: 271-275
PDF size: 226.92 Kb.


ABSTRACT

This work was aimed at evaluating the activity of native phosphate solubilizing bacteria on growth and development of radish (Raphanus sativus L.) plants. Bacteria were isolated from the Departament of Sucre (Colombia) soil using selective media (NBRIP), further identified by macroscopic and microscopic observation and biochemically characterized using the API System. Their ability to solublize phosphate was then evaluated using the vanadomolybdate acid phosphate colorimetric method. The microorganisms of the highest phosphate solubilizing capacity (Enterobacter sp. and Klebsiella sp.) were multiplied up to concentrations of 106, 107 and 108 c.f.u./mL and then evaluated on radish seeds. Eight treatments were used including witness control and commercial control. Biometric variables, root length, leaf area and dry weight, for treatments T5 (biopreparation with Klebsiella sp., 107 c.f.u./mL) and T1 (biopreparation with Enterobacter sp., 106 u.f.c./mL) were statistically higher than the control treatment. In addition, no significant differences regarding dry weight were found with respect to T7 (chemical fertilizer). The growth and development of radish plants were improved in treatments inoculated with bacteria when compared to controls, confirming the positive effect of phosphate solubilizing activity of the native strains.


REFERENCES

  1. Cheng-Hsiung C, Shang-Shyng Y. Thermo-tolerant phosphate-solubilizing microbes for multi-functional biofertilizer preparation. Bioresour Technol. 2009;100(4):1648-58.

  2. Conney M. Microbiología del suelo: Un enfoque exploratorio. Madrid: Paraninfo; 2000.

  3. Shenoy V, Kalagudi G. Enhancing plant phosphorus use effi ciency for sustainable cropping. Biotechnol Adv. 2005;23(7-8): 501-13.

  4. Glick BR. The enhancement of plant growth by free-living bacteria. Can J Microbiol. 1995;41:109-17.

  5. Mejía G. Agricultura para la vida: movimientos alternativos frente a la agricultura química. Cali: Feriva; 1995.

  6. Echeverri R, Castilla A. Biofertilizantes como mejoradores del proceso de nutrición del arroz. Rev Arroz. 2008;56(474): 12-27.

  7. Berc J, Muñoz O, Calero B. Vermiculture offers a new agricultural paradigm. Biocycle. 2004;45(6):56-7.

  8. Tognetti C, Laos F, Mazzarino MJ, Hernández MT. Composting vs. vermicomposting: A comparison of end product quality. Compost Sci Util. 2005;13(1):6-13.

  9. Christy M, Ramalingam R. Vermicomposting of sago - industrial solid waste using an epigeic earthworm Eudrilus eugeniae and macronutrients analysis of vermicompost. Asian J Microb Biotechnol Environ Sci. 2005;7(3):377-81.

  10. Trutmann P. Guía Salud de suelos. Manual para el cuidado de la salud de suelos. Para Agricultores, Promotores y extensionista. Honduras: PROMIPAC; 2001.

  11. Rodríguez H, Rubiano ME. Aislamiento e identifi cación de hongos solubilizadores de fosfatos aislados de cultivos de arroz y evaluación del pH y concentraciones de sacarosa y cloruro de sodio sobre su actividad solubilizadora [Tesis de Grado]. Bogotá: Pontifi cia Universidad Javeriana; 2002.

  12. Cordero J, Ortega-Rodés P, Ortega E. La inoculación de plantas de Pantoea sp., bacteria solubilizadora de fosfatos, incrementa la concentración de P en los tejidos foliares. Rev Colomb Biotechnol. 2008;10(1):111-21.

  13. Kloepper JW, Lifshitz R, Zablotowicz RM. Free-living bacterial inocula for enhancing crop productivity. Trends Biotechnol. 1989;7(2):39-44.

  14. Kim KY, Jordan D, McDonald GA. Effect of phosphate solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity. Biol Fert Soils. 1998;26(2):79-87.

  15. Oviedo ME, Iglesias MC. Utilización de bacterias solubilizadoras de fósforo en cultivo de raygrás [Abstract]. Comunicaciones Científi cas y Tecnológicas. Resumen A-053. Corrientes: Universidad Nacional del Nordeste, Argentina; 2005 [cited 2013 Aug 14]. Available from: http://www.unne. edu.ar/unnevieja/Web/cyt/com2005/5- Agrarias/A-053.pdf

  16. Nautiyal CS. An effi cient microbiological growth medium for screening phosphate solubilizing microorganism. FEMS Microbiol Lett. 1999;170(1):265-70.

  17. Kitson RE, Mellon MG. Colorimetric determination of phosphorus as molybdovanado phososphoric acid. Ind Eng Chem Anal Ed. 1944;16(6):379-83.

  18. Molano A. Aislamiento de bacterias biofertilizantes (Nitrobacter spp., Rhizobium spp., Azospirillum spp.) para un sistema de compost tipo windrow. Umbral Científi co. 2004;(5):25-32.

  19. Breed RS, Murray EGD, Smith NR, et al. Bergey’s Manual of determinative bacteriology. 7th ed. Baltimore: William & Wilkins Co.; 1957.

  20. Hernández A. Obtención de un biopreparado a partir de rizobacterias asociadas al cultivo de maíz (Zea mays L.) [Tesis de Doctorado en Ciencias Biológicas]. Facultad de Biología, Universidad de La Habana; 2002.

  21. ApiwebTM [Internet]. bioMérieux Clinical Diagnostics. Marcy l’Etoile: bioMerieux S.A.; c1996-2013 [cited 2013 Aug 8]. Available from: http://www.biomerieuxdiagnostics. com/servlet/srt/bio/clinicaldiagnostics/ dynPage?doc=CNL_PRD_CPL_ G_PRD_CLN_12

  22. Ramírez PR, Pérez MI. Evaluación del potencial de los biosólidos procedentes del tratamiento de aguas residuales para uso agrícola y su efecto sobre el cultivo de rábano rojo (Raphanus sativus L.). Rev Fac Nal Agr Medellín. 2006;59(2):3543-56.

  23. Coraspe HM, Tejera S. Procedimiento para toma de muestra de suelo. Revista Fonaiap Divulga. 1996;(54):23-5.

  24. López M, Martínez R, Brossard FM, Bolívar A, Alfonso N, Alba A, et al. Efecto de biofertilizantes bacterianos sobre el crecimiento de un cultivar de maíz en dos suelos contrastantes venezolanos. Agronomía Trop. 2008;58(4):391-401.

  25. Fernández LA, Zalba P, Gomez MA, Sagardoy MA. Bacterias solubilizadoras de fosfato inorgánico aisladas de suelos de la región sojera. Cienc Suelo. 2005; 23(1):31-7.

  26. Gupta R, Singal R, Shankar A, Kuhad RC, Saxena RK. A modifi ed plate assay for screening phosphate solubilizing microorganisms. J Gen Appl Microbiol. 1994; 40:255-60.

  27. Hewitson JF, Price R. Plant mineral nutrition in classroom: the radish, Raphanus sativus L. is a good plant for such studies. School Sci Rev. 1994;76(274):45-55.

  28. Mayak S, Tirosh T, Glick B. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol Biochem. 2004;42(6):565-72.

  29. Santillana N. Producción de biofertilizantes utilizando Pseudomonas sp. Ecol Apl. 2006;5(1-2):87-91.

  30. Martínez SM, Martinez GA. Effects of phosphate-solubilizing bacteria during the rooting period of sugar cane (Saccharum officinarum), Venezuela 51-71 variety, on the grower’s oasis substrate. In: Velázquez E, Rodríguez-Barrueco C, editors. First International Meeting on Microbial Phosphate Solubilization, Salamanca, Spain, 16-19 July 2002. Dordrecht: Springer; 2007. p. 317-23.

  31. Valero NO. Potencial biofertilizante de bacterias diazotrófi cas y solubilizadoras de fosfatos asociados a cultivos de arroz (Oryza sativa L.) [Tesis de Maestría Interfacultades en Microbiología]. Bogotá: Universidad Nacional de Colombia; 2003.

  32. Haahtela K, Rönkkö R, Laakso T, Williams PH, Korhonen TK. Root-associated Enterobacter and Klebsiella in Poa pratensis: characterization of an iron-scavenging system and a substance stimulating root hair production. Mol Plant-Microbe Interact. 1990;3:358-65.

  33. El-Khawas H, Adachi K. Identifi cation and quantifi cation of auxins in culture media of Azospirillum and Klebsiella and their effect on rice roots. Biol Fert Soils. 1999;28(4):377-81.

  34. Steenhoudt O, Vanderleyden J. Azospirillum, a free-living nitrogen-fi xing bacterium closely associated with grasses: genetic, biochemical and ecological aspects. FEMS Microbiol Rev. 2000;24(4): 487-506.

  35. Kämpfer P, Ruppel S, Remus R. Enterobacter radicincitans sp. nov., a plant growth promoting species of the family Enterobacteriaceae. Syst Appl Microbiol. 2005; 28(3):213-21.

  36. Madigan MT, Martinko JM, Parker J, editors. Brock: Biología de los microorganismos. 10 ed. Madrid: Pearson-Prentice Hall; 2003.

  37. Prescott LM, Harley JP, Klein DA. Microbiología. 5 ed. Madrid: McGraw-Hill Interamericana; 2004.

  38. Microbiología General [Internet]. Granada: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada; c2005. [cited 2013 Aug 14]. Available from: http://www.ugr.es/ ~eianez/Microbiologia/index.htm

  39. Bashan Y, De-Bashan LE. Protection of tomato seedlings against infection by Pseudomonas syringae pv. tomato by using the plant growth-promoting bacterium Azospirillum brasilense. Appl Environ Microbiol. 2002;68(6):2637-43.

  40. Lara C, Esquivel Avila LM, Negrete Peñata, JL. Bacterias nativas solubilizadores de fosfatos para incrementar los cultivos en el departamento de Córdoba-Colombia. Rev Bio Agro. 2011;9(2):114-20.




2020     |     www.medigraphic.com