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Instituto Nacional de Neurología y Neurocirugía

2013, Number s1

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Arch Neurocien 2013; 18 (s1)

Role of regulatory T cells on murine experimental Taenia crassiceps Cysticercosis

Ruiz-Monroy NM, López-Roblero A, Nájera-Ocampo M, Camacho-Vázquez C, Arce-Sillas A, Casanova-Hernández D, Palafox-Fonseca H, Melo-Salas M, Fragoso G, Sciutto-Conde E, Adalid-Peralta L

Language: Spanish
References: 32
Page: 33-37
PDF size: 192.65 Kb.


ABSTRACT

Objective: to explore the role of T regulatory (Treg) cells during infection by Taenia crassiceps in C57BL/6 mice. Materials and methods: female C57BL/6J, 4-5-weeks-old mice were used. Mice were distributed in two groups. Mice from group I were intraperitoneally inoculated with 20 cysticerci each. Mice from group II were not infected and were used as control. At different times post-infection (5, 30, 90, and 130 days) Treg cells level was evaluated at peritoneum, mesenteric ganglia, Peyer’s patches, and spleen. Concurrently, the proliferative capacity of spleen cells was assessed, by stimulating them both with Concanavalin A (ConA) and T. crassiceps antigens. Results: treg levels at the site of parasite establishment (peritoneum) were similar in the control group and the infected group during the period of study. Likewise, similar proliferation levels were observed in both groups when cells were stimulated with Con A. Cysticercal specific antigens failed to induce T effector or Treg cell proliferation. These results show that T. crassiceps promotes a decrease in the proliferative capacity of T lymphocytes, which does not depend on antigen-specific Tregs. Conclusions: the resistance to infection by T. crassiceps cysticerci in the strain C57BL/6 is not associated to the presence of Treg cells.


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